Cell Line Information:
Designations: MRC-5
Depositors: JP Jacobs
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Organ: lung
Disease: normal
Cell Type: fibroblast
Isolation date: September, 1966
Applications: transfection host; viruscide testing
Isoenzymes: G6PD, B
Age: 14 weeks gestation
Gender: male
Ethnicity: Caucasian
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence.
The Transfection Reagent:
MRC-5 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MRC-5 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MRC-5 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MRC-5 Cell Avalanche™ Transfection Reagent for transfection on MRC-5 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into MRC-5 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on WI-38 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for WI-38 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in MRC-5 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as MRC-5 Cell Avalanche Transfection Reagent. |
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| FIG. 2. MRC-5 cells were transfected with GFP vector (pEGFP-N3) by using MRC-5 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection. |
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For Other Cells: |
MRC-5 Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
293 Cell
WI-38 Cell
COS-7 Cell
BHK-21 Cell
3197-3 Cell
NIH/3T3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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