Cell Line Information:
Depositors: MG Brattain
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: colorectal carcinoma
Cellular Products: carcinoembryonic antigen (CEA) 1 ng per 10 exp6 cells per 10 days; keratin
Applications: transfection host
Tumorigenic: Yes
Age: adult
Gender: male
Comments: The cells are positive for keratin by immunoperoxidase staining. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. This line has a mutation in codon 13 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon
HCT 116 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
HCT 116 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HCT 116 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HCT 116 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of HCT 116 Cell Avalanche™ Transfection Reagent for transfection on HCT 116 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in HCT 116 Cells.
- Specifically optimized to deliver nucleic acids into HCT 116 Cells
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The ye |
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