Transfection-EZ Biosystems: Cell Lines
Avalanche®- Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
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PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)
PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)
EZ Biosystems
Description
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Cell Line Information:
Designations: PC-3
Organism: Homo sapiens (human)
Tissue: adenocarcinoma; prostate; metastatic site: bone
Age: 62 years
Tumor Stage: grade IV
Gender: male
Ethnicity: Caucasian
Morphology: epithelial
Growth properties: adherent
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells
AntigenExp: HLA A1, A9
Growth Properties: monolayer; the cells form clusters in soft agar and can be adapted to suspension growth
Depositors: M.E. Kaighn
The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
PC-3 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
PC-3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PC-3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PC-3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of PC-3 Cell Avalanche™ Transfection Reagent for transfection on PC-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
References:
Akamatsu S, Wyatt AW, Lin D, Lysakowski S, Zhang F, et al. The Placental Gene PEG10 Promotes Progression of Neuroendocrine Prostate Cancer.Cell Rep. 2015 Aug 11;12(6):922-36. PubMed PMID: 26235627.
Features:
- Specifically optimized to deliver nucleic acids into PC-3 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this PC-3 Cell Avalanche Transfection Reagent. |
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| FIG. 2. PC-3 cells were transfected with GFP vector (pEGFP-N3) by using PC-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection. |
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| For Other Cells: |
| PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.
DU 145 Cell 22Rv1 Cell VCaP Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
| Research Use Only. Not for use in diagnostic or therapeutic procedures. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200