ConviFlex™ RT-Taq Mix
Universal One-Step RT-PCR Mix for RT-qPCR
Indication
ConviFlex™ RT-Taq Mix is a lyophilized universal RT-PCR master mix, designed for the qualitative detection and analysis of RNA molecules, particularly suitable for detection of RNA-based viruses and functionality.
Principle of the Method
ConviFlex™ RT-Taq Mix is a ready-to-use one-step RT-PCR kit containing a MuLV reverse transcriptase, a hot start Taq polymerase, and dNTPs in a lyophilized master mix. After reconstitution with the supplied 2x rehydration buffer, ConviFlex™ RT-Taq Mix already consists of all core components required for RT-PCR amplification in one step, except for the RNA template and primers. This allows to greatly reduce the number of pipetting steps required to set up the RT-PCR reaction, the hands-on time, and the risk of cross-contaminations.
The Taq polymerase included in ConviFlex™ RT-PCR Mix has a hot start functionality. Its activity is blocked through anti-Taq DNA polymerase antibodies during the PCR set up even at ambient temperatures. This inhibition is completely reversed during the denaturation w hen the temperature increases above 70 °C, resulting in the activation of the Taq polymerase. Thus, the formation of unspecific PCR products and primer-dimers is strongly suppressed, resulting in PCR reactions with higher specificity, increased sensitivity, and greater yields. The reverse transcriptase included in the mix is a genetically modified from murine leukemia virus (M-MuLV).
ConviFlex™ RT-PCR Mix can easily be adapted to most RT-PCR protocols, programs and cycler types. For this reason, the RT-PCR set up and protocols in this document are only recommendations meant to assist you in the use of the RT-PCR system. Optimal volumes, concentrations, temperatures and incubation time may greatly vary for your specific application and should be adjusted to the requirements of your own assay.
Reagents
Each kit contains reagents for 25, 100, and 250 reactions (25 reactions / vial). The expiry date of the unopened package is marked on the package label. Lyophilized ConviFlex™ RT-Taq Mix should be stored at 2 - 8 °C, and after rehydration at ≤ -18 °C.
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