Rapid Micronucleus MoA Test
Kit for detection of Micronuclei, Clastogens, Aneugens and other
chromosomal Aberrations like NPBs, NBUDs - OECD TG487, OECD TG473
Rapid Micronucleus MoA (Mode of action)
is a ready to use kit for the fast identification of chromosomal
breakage (clastogenic effect) or damage to the spindle apparatur
(aneugenic effect) in dividing mammalien cells
(primary cells like human
lymphocytes or TK6 cell line). The test kit was developed to detect the
genotoxic potential of chemical substances.
The ready to use test kit Rapid Micronucleus MoA detects aberrations like micronuclei (MN), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), aneugens and clastogens according to OECD TG473 or OECD TG487.
Nucleoplasmic bridges and nuclear buds have been shown to be a
associated with chromosomal instability and chromosomal aberrations.
Cells are prepared as per standard
protocols according to OECD TG 487 or TG 473 and the slides are
sequentially treated with a series of solutions provided with the kit
“Rapid Micronucleus MoA”, including a human-specific, highly sensitive
centromere probe and a counterstaining solution containing DAPI
(4’,6-diamidino-2-phenylindole). Slides are sequentially washed with PBS
and an ethanol series after treatment. Centromere probes are then added
to the area of interest and the slide is heated at 80°C for 3 minutes
and transferred to a humidified chamber for 20 minutes. Washes and a
5-minutes counterstaining step follow prior to the addition of the
mounting solution.
Using a 40× magnification objective it
will be possible to easily identify micronuclei and assess whether a
centromere is present. Automated reading and archiving can be obtained
with the slide scanning systems, e.g. Metafer. Slides are usually ready
for evaluation within 1 hour. The application of FISH probes allows
directly to distinguish micronuclei originating either from chromosome
loss (aneugenic compounds) or breakage (clastogenic compounds).
FISH probe technology as used in the
Rapid Micronucleus MoA Kit can also be used for cytogenetic analysis in
research or in physical dosimetry, i.e. people exposed in medical or
natural radiation.
Characteristics for the Rapid Micronucleus MoA Test Kit
- 1 hour FISH Assay
- High specificity – no false positive results due to RNA, low-density micronuclei, precipitates, cytoplasmic alterations
- Ready to use kit including pan-centromeric probes, DAPI and reagents
- Simultaneous DAPI Counterstain
- Fast, clear identification of mode of action: NBUDs, NPBs, Aneugens or Clastogens
- In compliance with OECD TG473, TG487
- Can be used for Chemicals, Pharmaceuticals, Cosmetics, environmental samples
- For all human cell lines and whole blood
- Can be applied also for cytogenetic studies or in physical dosimetry
Other products and services
References
- Cuceu C, Colicchio B, Jeandidier E,
Junker S, Plassa F, Shim G, Mika J, Frenzel M, Al Jawhari M., Hempel WM,
O'Brien G, Lenain A, Morat L, Girinsky T, Dieterlen A, Polanska J,
Badie C, Carde P, M'Kacher R. 2018. Independent mechanisms lead to
genomic instability in Hodgkin lymphoma: Microsatellite or Chromosomal.
Cancers (Basel). 10(7).pii:E233. Erratum in: 2019. Cancers (Basel).
11(6).pii:E757.
- Zaguia N., Laplagne E., Colicchio
B., Cariou O., Al Jawhari M., Heidingsfelder L., Hempel WM, Bel Hadj
Jrad B, Jeandidier E, Dieterlen A, Carde P, Voisin P, M’Kacher R. 2020. A
new tool for genotoxic risk assessment: Reevaluation of the cytokinesis
block micronucleus assay using semi-automated scoring following
telomere and centromere staining. Mutat Res Gen Tox En. 850-851:503143.
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