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CRISPR-Cas 9: Reagents for CRISPR-Cas9

Reagents and kits used in CRISPR/Cas 9

T7-Scribe™Standard RNA IVT kit

T7-Scribe and SP6-Scribe Standard RNA IVT kits are specially formulated to utilise high concentrations of NTPs that are inhibitory to other in vitro transcription systems. For the production of in vitro transcribed RNA with T7/SP6 RNA polymerase (depending on kit) and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours.

CellScript

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
C-AS3107	T7-Scribe™ Standard RNA IVT kit	50 Reactions	£208.00	Quantity	Add to Order

Description

The T7-Scribe™ Standard RNA IVT Kit is specially formulated to enable users to obtain the maximum possible yields of canonical (GAUC) RNA from an in vitro transcription (IVT) reaction. The standard 2 hour, 20 μl reaction will yield up to 150 μg of RNA from 1 μg of the control template. These yields are made possible by the high-performance properties of the T7-Scribe enzyme.

The T7-Scribe Standard RNA IVT Kit produces exceptionally high yields of either long or short transcripts. The standard reaction can be scaled up to produce milligram amounts of RNA. In addition, a T7 -Scribe reaction can be readily modified to prepare fluorescent-, biotinylated- or digoxigenin-labelled RNA.

T7-Scribe IVT RNA can be processed into mRNA (5'-end capped and 3'-end poly(A) tailed) through the use of CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus™ Poly(A) Polymerase Tailing Kit (available separately).

See also SP6-Scribe Standard RNA IVT kit

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References

1.Karikó, K. et al., (2008) Molecular Therapy 16, 1833.

2.Anderson, B.R. et al., (2010) Nucl. Acids Res. 17, 5884.

3.Karikó, K. et al., (2005) Immunity 23, 165.

4.Karikó, K. and Weissman, D. (2007) Curr. Opin. Drug Discov. Devel. 10, 523.

5.Sambrook, J. et al., (1989) Molecular Cloning: A Laboratory Manual (2nd ed.), New York, Cold Spring Harbor Laboratory Press