Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Reagents, Labelling

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sulfoCyanine 3 NHS Ester

sulfoCyanine 3 NHS Ester (1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)prop-2-enylidene]indole-5-sulfonic acid, potassium salt)

Glen Research

Catalogue No.DescriptionPack SizePriceQty
50-5913-22sulfoCyanine 3 NHS Ester3.8mg £145.00 Quantity Add to Order

Description

  • Selectively reacts with primary aliphatic amine groups
  • Preparation of oligos with fluorophores sensitive to basic deprotection conditions

Cyanine 3 is a popular indocyanine fluorophore that exhibits low, non-specific binding and relatively pH-independent fluorescence. This NHS ester version allows for post-deprotection labeling, and unlike the phosphoramidite version, contains 2 sulfonate substitutions directly on the indocyanine nuclei. The sulfonates make the Cyanine 3 less susceptible to aggregation and do not significantly change the fluorescent properties of the dye.

 

sulfoCyanine 3 NHS Ester

Formula:C34H38KN3O10S2
M.W.:751.91
F.W.:598.73

Usage

  • Coupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 3.8 mg of the sulfoCyanine 3 NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt.
Specifications
Diluent Water, DMSO, DMF
StorageFreezer storage, -10 to -30°C, dry

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References

SDS and other references can be found on Glen Research's website here

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Applications & Benefits

Immunofluorescence, qPCR, Sanger sequencing

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