- Selectively reacts with primary aliphatic amine groups
- Preparation of oligos with fluorophores sensitive to basic deprotection conditions
Cyanine 5 is a popular indocyanine
fluorophore that exhibits low, non-specific binding and relatively
pH-independent fluorescence. This NHS ester version allows for
post-deprotection labeling, and unlike the phosphoramidite version,
contains 2 sulfonate substitutions directly on the indocyanine nuclei.
The sulfonates make the Cyanine 5 less susceptible to aggregation and do
not significantly change the fluorescent properties of the dye.
Usage
- Coupling:
For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve
3.9 mg of the sulfoCyanine 5 NHS Ester in 100 µL DMSO (or DMF). Dissolve
the amino-modified oligonucleotide in 500 µL 0.1 M
carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution
and vortex. Let react for 60 min at room temperature and desalt.
| Specifications |
| Diluent |
Water, DMSO, DMF |
| Storage | Freezer storage, -10 to -30°C, dry |
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