Quasar® 570-dT Phosphoramidite
5'-Dimethoxytrityloxy-5-[(N-(6-(N1-ethyl-3,3,3',3'-tetramethylindocarbocyanin-1'-yl)
hexanoyl)-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite, hexafluorophosphate salt
Product Specifications
Formula:
C79H100F6N8O10P2
Safety Data Sheet
Quasar®570 and Quasar®670 dyes are
two cyanine derivatives of Cyanine 3 and Cyanine 5, respectively. These
two fluorophores differ in structure simply by the number of carbons in
the conjugated polyene linkage. Cyanine dyes are a popular
oligonucleotide modification and are commonly used for imaging, flow
cytometry, and genomic applications. With Quasar®570-dT and
Quasar®670-dT, these dyes can be incorporated internally without
disrupting base pairing or stacking. Monomers can be inserted as a
replacement for a dT residue.
Particularly for Quasar® 670-dT, the use of UltraMILD monomers is
preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG:
10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group
on the dG with acetyl, use the UltraMILD Cap Mix A
(40-4210-xx/40-4212-xx). 0.02M Iodine for oxidation is recommended.
Details
Usage
- Coupling: 10 minute coupling time recommended. Use 0.02M Iodine to avoid degradation of the cyanine dye.
- Deprotection:
If dmf-dG is used during synthesis, deprotect in Ammonium Hydroxide for
2 hours at 65 °C; with ibu-dG, deprotect for 24-36 hours at room
temperature. Note Quasar? 570 is compatible with UltraFast deprotection
(AMA 10 minutes at 65 °C) if Ac-dC was used.
| Specifications |
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability |
1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-5953-95 |
50 µmol |
0.08 |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
| 10-5953-90 |
100 µmol |
0.15 |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-5953-02 |
0.5 g |
0.25 |
1.67 |
42.33 |
25.4 |
15.88 |
11.55 |
8.47 |
2.12 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-5953-95E |
50 µmol |
0.08 |
0.75 |
0.07 |
8.6 |
5.38 |
3.91 |
0.54 |
| 10-5953-90E |
100 µmol |
0.15 |
1.5 |
0.07 |
23.6 |
14.75 |
10.73 |
1.48 |
| 10-5953-02E | 0.25 g | 0.25 | 2.49 | 0.07 | 43.4 | 27.13 | 19.73 | 2.71 |
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