Description
Convertible
F-dC
The convertible nucleoside strategy is one of the most versatile methods
for producing modifications in bases to examine their effects on DNA
structure and activity. In some cases, with versatility comes difficulty
in that the convertible base is modified after oligonucleotide
synthesis. The chemistry is sometimes complex and base composition
analysis of the final oligonucleotide is required to verify structure.
The convertible dU monomer can be used to introduce a variety of
modifications at the convertible position, including N, O and S
modifications. Convertible F-dC is by far the simplest approach to the
preparation of oligonucleotides containing F-dC - normal ammonium
hydroxide treatment effects the conversion to F-dC. Convertible dA has
been used to prepare oligonucleotides containing multiple points for
attachment to solid supports. In this way, high capacity affinity
supports for the purification of DNA binding proteins have been
prepared. 2-F-dI is a convertible nucleoside for the preparation of
2'-dG derivatives following the displacement of the 2-fluorine by
primary amines.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions |
|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm |
|---|
| 10-1016-02 | 0.25 g | .25grams | 2.88 | 82.67 | 49.6 | 31 | 22.55 | 16.53 | 4.13 |
| 10-1016-90 | 100 µmol | .087grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions |
|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm |
|---|
| 10-1016-02 | 0.25 g | .25grams | 4.3 | 0.07 | 79.6 | 49.75 | 36.18 | 4.98 |
| 10-1016-90 | 100 µmol | .087grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |