2’-OMe-N6-Me-A, commonly referred to as m6Am, is a
crucial component of the 5’-cap of mRNA. 2’-OMe-A is often the first
transcribed nucleotide, and this modification is a result of methylation
at the N6-position of the nucleobase. The 5’-cap structures initiate
translation and protect RNA from degradation. Internal m6Am,
have also been implicated in regulating gene expression, RNA splicing,
and cancer proliferation. We offer the 2’-OMe-N6-Me-A RNA monomer with a
phenoxyacetyl protecting group to minimize potential branching.
2’-OMe-N6-Me-A-CE Phosphoramidite is compatible with popular synthesis
and deprotection methods, from UltraMild to AMA.
 Phosphoramidite fig 2.png)
Product Specifications
F.W.: 357.28
Usage
- Coupling:
6 minute coupling time. To avoid any exchange of the iPr-Pac group on
the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
- Deprotection:
UltraMild deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours
at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium
Hydroxide.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-3105-95 |
50 µmol |
0.47 |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
| 10-3105-90 |
100 µmol |
0.93 |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-3105-02 |
0.25 g |
0.25 |
2.68 |
0 |
45.6 |
28.5 |
20.73 |
15.2 |
3.8 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-3105-95E |
50 µmol |
0.47 |
0.75 |
0.07 |
8.6 |
5.38 |
3.91 |
0.54 |
| 10-3105-90E |
100 µmol |
0.93 |
1.5 |
0.07 |
23.6 |
14.75 |
10.73 |
1.48 |
| 10-3105-02E |
0.25 g |
0.25 |
4 |
0.07 |
73.6 |
46 |
33.45 |
4.6 |
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