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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound.

The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells.

Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request.

•Ultrapure quality

•High activity

•Wide range

•Lyophilized powder

•Custom lectins and conjugates

Trichosanthes japonica agglutinin II (TJA-II)

TJA-II is isolated from the root tubers of Japanese gourd. It is highly purified and one purification step involves bio specific affinity chromatography to ensure highest possible binding affinity. TJA-II has affinity for lactose favouring fucose a1->2 lactose.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC- 05-0033-2Trichosanthes japonica agglutinin II (TJA-II)2 mg £292.00 Quantity Add to Order
MC- 05-0033-25Trichosanthes japonica agglutinin II (TJA-II)25 mg £809.00 Quantity Add to Order
MC-05-0033-100Trichosanthes japonica agglutinin II (TJA-II)100 mg £2,427.00 Quantity Add to Order

Description

Trichosanthes japonica agglutinin II (TJA-II)

 

Features

• Affinity for lactose
• Ultrapure quality

 

Applications

• Anti-H agglutinin (TJA-II)

 

Product Description

TJA-II is isolated from the root tubers of Japanese gourd. It is highly purified and one purification step involves bio specific affinity chromatography to ensure highest possible binding affinity. TJA-II has affinity for lactose favouring fucose a1->2 lactose.

Storage and Stability

Stable for at least 3 years from production date when stored below -20 °C. May be shipped at ambient temperature. After reconstitution in PBS pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

 

Documents

Product Sheet

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

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