NAT-MAT® All-in-One System
NAT-MAT® All-in-One System provides all necessary components, including cells, extraction system, and dPCR assay to perform monocyte activation test (MAT) in order to detect pyrogen contaminations in pharmaceuticals.
The NAT-MAT® system relies on the use of NAT-MAT® Cells which are stimulated with a pyrogen standard and the sample to be tested followed by nucleic acid extraction (NAT-MAT® Extraction) and subsequent dPCR analysis of the gene expression of IL-1β and TNF-α (NAT-MAT® dPCR). By simultaneously measuring the gene expression of a housekeeping gene, the pyrogenic load can be normalized to the cell number, which allows highly accurate results.
Components
- NAT-MAT® Cells (Cat. No. 58-0501)
- NAT-MAT® Extraction (Cat. No. 58-0502)
- NAT-MAT® dPCR (Cat. No. 58-0503)
Package Size
Cat. No. 58-0511: 96 reactions
Result Evaluation
Absolute Quantification
Required Lab Device and Consumables
NAT-MAT® Cells:
- Liquid nitrogen or ≤-150 °C ultra-freezer
- CO2 Incubator
- Laminar airflow cabinet
- Water bath
- Centrifuge (for 15 ml falcons)
- 15 ml falcon tubes
- Fetal bovine serum
- Inverted microscope (optional)
- Reference standard endotoxin (RSE), e.g. preparations from USP or EP (BRP)
- Sterile, non-pyrogenic water (LAL water)
- 2 relevant non-endotoxin pyrogens (optional)
- Pyrogen-free glass tubes
- Microcentrifuge
- Vortex mixer
- Pipettes with corresponding filter tips (10 μl, 100 μl, and 1000 μl)
- Multi-channel pipette (optional)
NAT-MAT® Extraction:
- 1-propanol, ≥99,5 %, p.a.
- Automated extraction device (e.g. KingFisher™ Flex)
- 96-well plates for automated extraction device
- Important: For manual extraction (not recommended): thermal shaker and magnetic rack
NAT-MAT® dPCR:
- QIAcuity® digital PCR system
- QIAcuity® Nanoplate 8.5k 96-well
- QIAcuity® OneStep Advanced Probe Kit
- Nanoplate tray
- Nanoplate seal
- 1.5 ml reaction tubes
- Microcentrifuge for 1.5 ml PCR tubes
- Vortex mixer
- Pipettes with corresponding filter tips
- Pipetting robot (optional, e.g. QIAgility® with nanoplate adapter)
Shipment, Shelf Life, and Storage
The expiry date of the unopened package is marked on the package label.
NAT-MAT® Cells: Shipment with dry ice (cells) and ambient temperature (cell culture media and plate) in 2 separate packages. The cells must be stored at ≤-150 °C or liquid nitrogen until use. The cell culture media must be stored at 2 to 8°C until use.
NAT-MAT® Extraction: Shipment and storage at ambient temperature.
NAT-MAT® dPCR: Shipment at ambient temperature. The kit components must be stored at 2 to 8°C until use. The rehydrated components must be stored at ≤ -18°C for a maximum of one month.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200