Plasmid DNA Restriction Mapping Lab
Introduce students to the use of restriction enzymes for creating
plasmid maps. This activity uses pre-digested plasmid DNA samples ready
for gel electrophoresis and can be completed in a single class period!
Plasmid maps are useful diagrams that show the
structure and genetic features of plasmids – small, circular DNA
molecules used in molecular biology labs. These maps are essential tools
for planning cloning experiments, designing PCR strategies, selecting
appropriate restriction enzymes, and sharing plasmid information between
researchers. In this activity, students will learn how to use restriction enzymes to create their own plasmid maps.
The use of pre-digested plasmid DNA samples
ready for gel electrophoresis analysis means this activity can be
completed in a single class period.
- Techniques: Micropipetting, gel electrophoresis
- Topics: Restriction digestion analysis, DNA fragment size estimation
- Time required: Can be completed in one 45-minute class period
- Level: Advanced high school through college
What's included
DNA samples for at least 8 lab groups:
- BamHI Digested Plasmid (150 μl)
- ApaLI Digested Plasmid (150 μl)
- Double Digested Plasmid (150 μl)
Storage notes:
- If kept in the freezer, reagents can be stored for 12 months after receipt.
Required equipment:
Gel electrophoresis reagents and plastic tubes are supplied by the user
- Agarose
- Gel electrophoresis buffer (e.g.TBE)
- Fluorescent DNA stain (e.g. SeeGreen™ or GelGreen®)
- Plastic tubes for distributing samples to student groups
The Learning Lab Companion Kit (KT-1510-01) provides sufficient reagents to prepare and run eight gels when using the blueGel electrophoresis system, as well as plastic tubes to distribute samples to student groups.
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