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In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

PB/ß-Naphtoflavone induced hamster liver S9 Fraction

PB/ß-Naphtoflavone induced hamster liver S9 Fraction for Enhanced Ames Test

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
XPRS-H-PB01LIQPB/ß-Naphtoflavone induced hamster liver S9 Fraction1 ml £120.00 Quantity Add to Order
XPRS-H-PB03LIQPB/ß-Naphtoflavone induced hamster liver S9 Fraction3 ml £243.00 Quantity Add to Order
XPRS-H-PB05LIQPB/ß-Naphtoflavone induced hamster liver S9 Fraction5 ml £311.00 Quantity Add to Order

Description

Due to bacteria being unable to metabolize molecules, the mutagenic potential of samples is assessed directly and in the presence of metabolic activation system: Microsomal fraction S9 of Phenobarbital/ß-Naphtoflavone induced hamster liver homogenate. Hamster liver S9 is used preferentially in the Enhanced Ames Test (OECD 471) for Nitrosamines, or in ADME (pharmacokinetic studies) for pharmaceutical preparations.

Phenobarbital/ß-Naphtoflavone induced hepatic S9 is a buffered solution containing the post mitochondrial S9 fraction of a pool of livers from Golden Syrian Hamster. Liver S9 fractions (the 9000g supernatant of a liver homogenate) contain both microsomal and cytosolic fractions. S9 fraction includes many cytosolic enzymes: N-acetyl transferases, methyltransferases, sulfotransferases, glutathione transferases, aldehyde oxidase, xanthine oxidase. Both Phase I (Cytochrome P450-Monooxigenases) and Phase II metabolites act simultaneously on test samples. Due to the fact that the enzymes in the S9 fractions are diluted significantly, cofactors such as e.g. β-Nicotinamide adenine dinucleotide phosphate-regenerating system (NADPH, Phase I oxidation) are required for activity. Cofactors are added to the S9 preparation before the experiment (S9 Cofactor Kit, art.no. PCO-0800, PCO-1800).   

 

Characteristics

  • Protein Concentration: 25 - 35 mg/ml
  • High activities of Cytochrome P450: 1A2 (MROD), 1A1, 1A2 (EROD), 3A, 2B (BROD), 2B1, 2B2 (PROD)
  • Storage: -20 or -80 oC 
  • Shipment on dry ice from storage in Germany or from Switzerland
  • Shelf Life: 12 -24 months
  • In full compliance with OECD 471 guideline, ICH M7 
  • Requested volumes in different Ames test: 0.44 mL/strain/compound in MicroAmes24, 0.35 mL/strain/ compound in Ames MPF, 1.4 mL / strain/ compound in Ames agar plate Test

 

Also available

  • S9 Cofactor Kit, art.no. PCO-0800, PCO-1800
  • Non induced S9 Fractions from other animals

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