Endonuclease VIII
• Removes oxidatively damaged pyrimidines from DNA
• Has both β and δ lyase activity, whereas Endonuclease III only has β lyase activity
Endonuclease VIII Kit contains Endonuclease VIII enzyme (10 U/µL) and 10X Reaction Buffer
E.coli Endonuclease VIII is a bifunctional DNA glycosylase
with DNA N-glycosylase and AP lyase activities. The N-glycosylase
activity releases damaged pyrimidines from double-stranded DNA
generating an apurinic (AP) site. The AP lyase activity cleaves the DNA
phosphodiester backbone at AP sites via β- and δ-elimination, creating a
one nucleotide gap with 5′ and 3′ phosphate termini. Damaged bases
recognised and removed by Endonuclease VIII include thymine glycol,
uracil glycol, urea, 5,6-dihydroxythymine, 5-hydroxy-5-methylhydantoin,
5-hydroxy-6-hydrothymine, 5,6-dihydrouracil and alloxan.
SDS
Manuals and user guides
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200