NxGen T7 RNA Polymerase
Product Details
Supplied at 50,000 U/mL in 50 mM Tris-HCl,
100 mM NaCl, 1 mM dithiothreitol, 1 mM E
DTA, 50% glycerol, 0.1% Triton
X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer
which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C.
T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7
promoter. It is a DNA-dependent RNA polymerase cloned from the T7
bacteriophage, which recognises the T7 promoter and terminator sequences
with high specificity.
Unit Definition: One unit is defined as the amount
of enzyme that will incorporate 1 nmol of ATP into acid-precipitable
material in 1 hour at 37 °C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
| Unit Concentration |
50,000 U/mL |
| Purity (SDS-PAGE) |
>99% |
| SS Exonuclease |
500 U <0.1% released |
| DS Exonuclease |
500 U <0.1% released |
| Endonuclease |
500 U <10% converted |
| RNase Contamination |
500 U = none detected |
| E. coli 16S rDNA Contamination |
500 U <10 copies |
| Storage |
-20 °C |
SDS
Manuals and user guides
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200