RNase T1
Product Description:
Ribonuclease T1 (RNase T1) from Aspergillus oryzae specifically hydrolyzes the phosphodiester bond after guanosine bases in RNA. RNase T1 is not inhibited by ScriptGuard™ RNase Inhibitor, an RNase A-family inhibitor.
Due to its base-specific cleavage activity, RNase T1 can be used for the following applications:
- RNA mapping.
- RNA structure studies.
- RNA sequencing.
- Removal of RNA from DNA preparations.
Product Performance:
Figure 1. RNase T1 treatment results in base-specific
cleavage. 100 picomoles of 25-nt ssRNA oligo containing a single G
nucleotide at position 8 was treated with varying amounts of RNase T1
for 10 min at 37°C. RNase T1 cleavage is base-specific, with a 17-nt RNA
band visible following treatment (lanes 2 and 3).
Figure 2. RNase T1 fully degrades ribosomal RNA. 12 μg of E. coli ribosomal RNA was treated with varying amounts of RNase T1 for 10 min at 37°C. Treatment with
2 U (lane 2) and 1 U (lane 3) of RNase T1 resulted in complete degradation of the RNA.
Materials Supplied:
Important Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C.
|
Ribonuclease T1 (RNase T1) |
|
Component |
Volume |
RNase T1, 1,000 U/μl
in 50% glycerol, 50 mM Tris-HCl, pH 7.5, 0.1 M NaCl, 0.1 mM EDTA and 0.1% Triton® X-100. |
100 μl |
Manual
SDS
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