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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-A-CE Phosphoramidite

2'-OMe-A-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3100-022'-OMe-A-CE Phosphoramidite 0.25g £23.00 Quantity Add to Order
10-3100-052'-OMe-A-CE Phosphoramidite 0.5g £45.00 Quantity Add to Order
10-3100-102'-OMe-A-CE Phosphoramidite 1.0g £91.00 Quantity Add to Order
10-3100-902'-OMe-A-CE Phosphoramidite 100µmoles £14.00 Quantity Add to Order

Description

2'-OMe-A-CE Phosphoramidite

2’-OMe-RNA phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Structure

Catalog Number: 10-3100-xx

Description: 2'-OMe-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine,2'-O-methyl,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H54N7O8P M.W.: 887.97 F.W.: 343.24
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 DAYS

2’-Ome-rna phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

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Protocols

MSDS

Glen Report 6.2

Glen Report 6.1

Glen Report 19.2

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References

(1) B. Oberhauser and E. Wagner, Nucleic Acids Res., 1992, 20, 533-538.
(2) J. Goodchild, Nucleic Acids Res., 1992, 20, 4607-4612.
(3) C. Giovannangeli, N.T. Thuong, and C. Helene, Nucleic Acids Res., 1992, 20, 4275-4281.
(4) C. Giovannangeli, M. Rougee, T. Garestier, N.T. Thuong, and C. Helene, Proc. Natl. Acad. Sci. U.S.A., 1992, 89, 8631-8635.
(5) J.A. Doudna, J.W. Szostak, A. Rich, and N. Usman, J. Org. Chem., 1990, 55, 5547-5549.

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Notes

2'-OMe-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine,2'-O-methyl,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H54N7O8P M.W.: 887.97 F.W.: 343.24
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 DAYS
  Material Safety Data Sheet

Literature Highlights

Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA
Glen Report 6.1: 2'-OMe-RNA UPDATE
Glen Report 19.2: New Lines of Monomers - HT for High Throughput and LC for low cost RN

 

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3100-02 0.25grams .25grams 2.82 80.67 48.4 30.25 22 16.13 4.03
10-3100-05 0.5grams .5grams 5.63 174.33 104.6 65.38 47.55 34.87 8.72
10-3100-10 1.0gram 1grams 11.26 362 217.2 135.75 98.73 72.4 18.1
10-3100-90 100µmoles .089grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-3100-02 0.25grams .25grams 4.2 .067 77.6 48.5 35.27 4.85  
10-3100-05 0.5grams .5grams 8.4 .067 161.6 101 73.45 10.1  
10-3100-10 1.0gram 1grams 16.81 .067 329.8 206.13 149.91 20.61  
10-3100-90 100µmoles .089grams 1.5 .067 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-3100-02 0.25grams .25grams 4.2 .067 79.2 49.5 36    
10-3100-05 0.5grams .5grams 8.4 .067 163.2 102 74.18    
10-3100-10 1.0gram 1grams 16.81 .067 331.4 207.13 150.64    
10-3100-90 100µmoles .089grams 1.5 .067 25.2 15.75 11.45

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3100-02 0.25grams .25grams 2.82 80.67 48.4 30.25 22 16.13 4.03
10-3100-05 0.5grams .5grams 5.63 174.33 104.6 65.38 47.55 34.87 8.72
10-3100-10 1.0gram 1grams 11.26 362 217.2 135.75 98.73 72.4 18.1
10-3100-90 100µmoles .089grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3100-02 0.25grams .25grams 4.2 .07 77.6 48.5 35.27 4.85
10-3100-05 0.5grams .5grams 8.4 .07 161.6 101 73.45 10.1
10-3100-10 1.0gram 1grams 16.81 .07 329.8 206.13 149.91 20.61
10-3100-90 100µmoles .089grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3100-02 0.25grams .25grams 4.2 .07 79.2 49.5 36

10-3100-05 0.5grams .5grams 8.4 .07 163.2 102 74.18

10-3100-10 1.0gram 1grams 16.81 .07 331.4 207.13 150.64

10-3100-90 100µmoles .089grams 1.5 .07 25.2 15.75 11.45

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Related products

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