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Oligo Synthesis

Oligo Synthesis : CEPs

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5-Br-dC-CE Phosphoramidite

5-Br-dC-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1080-025-Br-dC-CE Phosphoramidite 0.25g £182.00 Quantity Add to Order
10-1080-905-Br-dC-CE Phosphoramidite 100µmoles £73.00 Quantity Add to Order

Description

5-Br-dC-CE Phosphoramidite

Structure

Catalog Number: 10-1080-xx

Description: 5-Br-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-5-bromo-2'-deoxyCytidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C46H51BrN5O8P M.W.: 912.82 F.W.: 368.08
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Mild deprotection: Ammonium Hydroxide for 24 hours at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 24 hours

HALOGENATED NUCLEOSIDES

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

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Protocols

MSDS

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Notes

Oligonucleotides containing a bromo or iodo group are prepared conventionally with the exception that deprotection is carried out in ammonium hydroxide at room temperature for 24 hours.  Under these conditions, degradation of the halogen group was less than 2%.

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Applications & Benefits

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1080 5-Br-dC 287 6

3.1

Frequently Asked Technical Question

QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?

RESPONSE:See: Table of Extinction Coefficients

REFERENCE(S):
B. Froehler, Gilead
M. Powell
R. Somers

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1080-02 0.25grams .25grams 2.74 78 46.8 29.25 21.27 15.6 3.9
10-1080-90 100µmoles .091grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1080-02 0.25grams .25grams 4.09 .07 75.4 47.13 34.27 4.71
10-1080-90 100µmoles .091grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1080-02 0.25grams .25grams 4.09 .07 77 48.13 35

10-1080-90 100µmoles .091grams 1.5 .07 25.2 15.75 11.45

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Related products

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