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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-Bromohexyl Phosphoramidite

5'-Bromohexyl Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1946-025'-Bromohexyl Phosphoramidite 0.25g £182.00 Quantity Add to Order
10-1946-905'-Bromohexyl Phosphoramidite 100µmoles £55.00 Quantity Add to Order

Description

5'-Bromohexyl Phosphoramidite

Structure

Catalog Number: 10-1946-xx

Description: 5'-Bromohexyl Phosphoramidite

6-Bromo-hex-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C15H30BrN2O2P M.W.: 381.29 F.W.: Bromide: 243.04 g/mol Azide: 205.15
Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling recommended.
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_10-1946.pdf). Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

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Protocols

MSDS

Glen Report

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1946-90 100µmoles .038grams 1 20 12 7.5 5.45 4 1
10-1946-02 0.25grams .25grams 6.56 205.33 123.2 77 56 41.07 10.27
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1946-90 100µmoles .038grams 1.5 .07 23.6 14.75 10.73 1.48
10-1946-02 0.25grams .25grams 9.79 .07 189.4 118.38 86.09 11.84
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1946-90 100µmoles .038grams 1.5 .07 25.2 15.75 11.45

10-1946-02 0.25grams .25grams 9.79 .07 191 119.38 86.82

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Related products

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