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Oligo Synthesis

Oligo Synthesis : CEPs

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5-F-dU-CE Phosphoramidite

5-F-dU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1092-025-F-dU-CE Phosphoramidite 0.25g £418.00 Quantity Add to Order
10-1092-905-F-dU-CE Phosphoramidite 100µmoles £136.00 Quantity Add to Order

Description

5-F-dU-CE Phosphoramidite

Structure

Catalog Number: 10-1092-xx

Description: 5-F-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5-fluoro-2'-deoxyUridine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C39H46FN4O8P M.W.: 748.79 F.W.: 308.16
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

HALOGENATED NUCLEOSIDES

Brominated and iodinated nucleosides are used in crystallography studies of oligonucleotide structure. They are also photolabile and are used for cross-linking studies to probe the structure of protein-DNA complexes. Antibodies exist to Br-dU and oligonucleotides containing Br-dU can be used as probes.

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Protocols

MSDS

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1092-02 0.25grams .25grams 3.34 98 58.8 36.75 26.73 19.6 4.9
10-1092-90 100µmoles .075grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1092-02 0.25grams .25grams 4.98 .07 93.2 58.25 42.36 5.83
10-1092-90 100µmoles .075grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1092-02 0.25grams .25grams 4.98 .07 94.8 59.25 43.09

10-1092-90 100µmoles .075grams 1.5 .07 25.2 15.75 11.45

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Related products

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