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Oligo Synthesis

Oligo Synthesis : CEPs

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6-thio-dG-CE Phosphoramidite

6-thio-dG-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1072-026-thio-dG-CE Phosphoramidite 0.25g £973.00 Quantity Add to Order
10-1072-906-thio-dG-CE Phosphoramidite 100µmoles £373.00 Quantity Add to Order
10-1072-956-thio-dG-CE Phosphoramidite 50µmoles £195.00 Quantity Add to Order

Description

Structure

Catalog Number: 10-1072-xx

Description: 6-thio-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-trifluoroacetyl-2'-deoxy-6-(2-cyanoethyl)thio-
Guanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C45H50F3N8O7PS M.W.: 934.97 F.W.: 345.26
Diluent: Anhydrous Acetonitrile
Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation.
Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 5hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs.
Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 24 hours

The C-nucleoside 2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets. 2-Aminopurine lacks groups critical for hydrogen bonding and is a mildly fluorescent base.

Demand for sulfur modified bases continues to expand for investigations of oligonucleotide structure, but primarily for cross-linking purposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications for photo cross-linking and photoaffinity labelling experiments. Oligos containing 2-thio-dT are useful in examining protein-DNA interaction by acting as photosensitizing probes. The thiocarbonyl group in 2-thio-dT is especially interesting in that it is available to react with compounds associating with the minor groove of DNA. 2-Amino-A forms a very stable base pair with T containing three hydrogen bonds but the stability of the base pair with 2-thio-T is greatly diminished. Due to steric interactions between the 2-thio group of thymidine and the 2-amino group of 2-amino-A, the base pair contains only a single hydrogen bond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity for natural oligonucleotides but show little affinity for other similar oligos even of a complementary sequence.

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Protocols

MSDS

Glen Report 11.1: MORE NOVEL MONOMERS

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Notes

6-thio-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-trifluoroacetyl-2'-deoxy-6-(2-cyanoethyl)thio-
Guanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C45H50F3N8O7PS M.W.: 934.97 F.W.: 345.26
Diluent: Anhydrous Acetonitrile
Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation.
Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 5hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours
  Material Safety Data Sheet

Literature Highlights

Glen Report 11.1: MORE NOVEL MONOMERS

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1072-02 0.25grams .25grams 2.67 75.67 45.4 28.38 20.64 15.13 3.78
10-1072-90 100µmoles .093grams 1 20 12 7.5 5.45 4 1
10-1072-95 50µmoles .047grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1072-02 0.25grams .25grams 3.99 .067 73.4 45.88 33.36 4.59  
10-1072-90 100µmoles .093grams 1.5 .067 23.6 14.75 10.73 1.48  
10-1072-95 50µmoles .047grams .75 .067 8.6 5.38 3.91 .54  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1072-02 0.25grams .25grams 3.99 .067 75 46.88 34.09    
10-1072-90 100µmoles .093grams 1.5 .067 25.2 15.75 11.45    
10-1072-95 50µmoles .047grams .75 .067 10.2 6.38 4.64
.

 

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1072-02 0.25grams .25grams 2.67 75.67 45.4 28.38 20.64 15.13 3.78
10-1072-90 100µmoles .093grams 1 20 12 7.5 5.45 4 1
10-1072-95 50µmoles .047grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1072-02 0.25grams .25grams 3.99 .07 73.4 45.88 33.36 4.59
10-1072-90 100µmoles .093grams 1.5 .07 23.6 14.75 10.73 1.48
10-1072-95 50µmoles .047grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1072-02 0.25grams .25grams 3.99 .07 75 46.88 34.09

10-1072-90 100µmoles .093grams 1.5 .07 25.2 15.75 11.45

10-1072-95 50µmoles .047grams .75 .07 10.2 6.38 4.64

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Related products

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