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Oligo Synthesis

Oligo Synthesis : CEPs

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7-Deaza-dA-CE Phosphoramidite

7-Deaza-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1001-027-Deaza-dA-CE Phosphoramidite 0.25g £1,105.00 Quantity Add to Order
10-1001-907-Deaza-dA-CE Phosphoramidite 100µmoles £382.00 Quantity Add to Order
10-1001-957-Deaza-dA-CE Phosphoramidite 50µmoles £200.00 Quantity Add to Order

Description

7-Deaza-dA-CE Phosphoramidite

Structure

Catalog Number: 10-1001-xx

Description: 7-Deaza-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-7-deaza-2'-deoxyAdenosine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H53N6O7P M.W.: 856.96 F.W.: 312.22

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

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Protocols

MSDS

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Applications & Benefits

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1001 7-deaza-dA 270 11.3

9.4

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1001-02 0.25grams .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-1001-90 100µmoles .086grams 1 20 12 7.5 5.45 4 1
10-1001-95 50µmoles .043grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1001-02 0.25grams .25grams 4.35 .07 80.6 50.38 36.64 5.04
10-1001-90 100µmoles .086grams 1.5 .07 23.6 14.75 10.73 1.48
10-1001-95 50µmoles .043grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1001-02 0.25grams .25grams 4.35 .07 82.2 51.38 37.36

10-1001-90 100µmoles .086grams 1.5 .07 25.2 15.75 11.45

10-1001-95 50µmoles .043grams .75 .07 10.2 6.38 4.64

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Related products

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