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Oligo Synthesis

Oligo Synthesis : CEPs

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8-oxo-dG-CE Phosphoramidite

8-oxo-dG-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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10-1028-028-oxo-dG-CE Phosphoramidite 0.25g €1,063.20 Quantity Add to Order
10-1028-908-oxo-dG-CE Phosphoramidite 100µmoles €387.60 Quantity Add to Order
10-1028-958-oxo-dG-CE Phosphoramidite 50µmoles €193.20 Quantity Add to Order

Description

8-oxo-dG-CE Phosphoramidite

Structure

Catalog Number: 10-1028-xx

Description: 8-oxo-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C44H54N7O9P M.W.: 855.93 F.W.: 345.21
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site.
Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 24 hours

DNA Damage/Repair

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

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Protocols

MSDS

Glen Report 7.1: NEW MINOR BASES

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Notes

Synthetic oligonucleotides containing 8-oxo-dG must be cleaved and deprotected with ammonium hydroxide containing 0.25M 2-mercaptoethanol to avoid oxidative degradation of 8-oxo-dG sites.

Frequently Asked Technical Question

QUESTION: How do you deprotect 8-oxo-dG?

RESPONSE:Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site

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Applications & Benefits

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1028 8-oxo-dG 294 5.2 250 6.7 5.9

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1028-02 0.25grams .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-1028-90 100µmoles .086grams 1 20 12 7.5 5.45 4 1
10-1028-95 50µmoles .043grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1028-02 0.25grams .25grams 4.36 .07 80.8 50.5 36.73 5.05
10-1028-90 100µmoles .086grams 1.5 .07 23.6 14.75 10.73 1.48
10-1028-95 50µmoles .043grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1028-02 0.25grams .25grams 4.36 .07 82.4 51.5 37.45

10-1028-90 100µmoles .086grams 1.5 .07 25.2 15.75 11.45

10-1028-95 50µmoles .043grams .75 .07 10.2 6.38 4.64

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Related products

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