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Oligo Synthesis

Oligo Synthesis : CEPs

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Ac-dC-Me Phosphonamidite

Ac-dC-Me Phosphonamidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1115-02Ac-dC-Me Phosphonamidite 0.25g £250.00 Quantity Add to Order
10-1115-05Ac-dC-Me Phosphonamidite 0.5g £500.00 Quantity Add to Order

Description

Ac-dC-Me Phosphonamidite

Structure

Catalog Number: 10-1115-xx

Description: Ac-dC-Me Phosphonamidite

5'-Dimethoxytrityl-N4-acetyl-2'-deoxyCytidine,3'-[(methyl)-
(N,N-diisopropyl)]-phosphonamidite
Formula: C39H49N4O7P M.W.: 716.81 F.W.: 287.21
Diluent: Anhydrous Acetonitrile
Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended.
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_Me-Phosphonamidites.pdf). Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

methyl phosphonamidite

Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages. However, deprotection and purification techniques differ and a description of the procedures is included with each delivery of these monomers. We also offer the dC monomer with acetyl base protection.1 This protecting group is removed with ammonium hydroxide during the cleavage step, eliminating modification at the dC sites during the deprotection step using ethylenediamine in ethanol.

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Protocols

MSDS

Glen Report 9.1: PROTECTING GROUPS FOR DNA, RNA AND 2'-OMe-RNA MONOMERS

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1115-02 0.25grams .25grams 3.49 103 61.8 38.63 28.09 20.6 5.15
10-1115-05 0.5grams .5grams 6.98 219.33 131.6 82.25 59.82 43.87 10.97
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1115-02 0.25grams .25grams 5.21 .07 97.8 61.13 44.45 6.11
10-1115-05 0.5grams .5grams 10.41 .07 201.8 126.13 91.73 12.61
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1115-02 0.25grams .25grams 5.21 .07 99.4 62.13 45.18

10-1115-05 0.5grams .5grams 10.41 .07 203.4 127.13 92.45

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Related products

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