| ATC Trimer Phosphoramidite |
Catalog Number: 10-1031-xx
Description: 5'-OMe-dT-CE Phosphoramidite
5'-O-methyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-
phosphoramidite |
| Formula: C20H33N4O6P |
M.W.: 456.48 |
F.W.: 318.22 |
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ Technical/TB_ABITransfer.pdf.
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| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.(Note: this product is a 5'-terminator.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
chain terminators
In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)
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