| EclipseTM Quencher Phosphoramidite |
Catalog Number: 10-5925-xx
Description: Eclipse™Quencher Phosphoramidite
4-N-methyl-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramdite |
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Glen Research’s agreement with ELITechGroup, formerly Epoch Biosciences,
allows us to offer several of their proprietary products designed for
the synthesis of novel DNA probes. We are pleased to offer products
based on ELITechGroup’s Redmond Red®, Yakima Yellow® and AquaPhluor® 593
fluorophores and Eclipse® non-fluorescent quencher. Under our agreement
we also supply PPG, a modified nucleoside, and 5’-Aldehyde-Modifier C2
Phosphoramidite. The fluorescent dyes, Yakima Yellow, Redmond Red and
AquaPhluor 593, are available as phosphoramidites and supports. Yakima
Yellow has an absorbance maximum at 530 nm and emission maximum at 549
nm, Redmond Red’s absorbance and emission maxima are at 579 nm and 595
nm, respectively, and AquaPhluor 593 has an absorbance maximum at 593 nm
and emission maximum at 613 nm. The fluorescein alternative, Gig Harbor
Green, has been discontinued.
The Eclipse quencher from ELITechGroup solves most of the problems
inherent in the synthesis of molecular beacon and FRET probes. The
Eclipse molecule is highly stable and can be used safely in all common
oligo deprotection schemes. The absorbance maximum for Eclipse Quencher
is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure
of the Eclipse Quencher is substantially more electron deficient than
that of dabcyl and this leads to better quenching over a wider range of
dyes, especially those with emission maxima at longer wavelengths (red
shifted) such as Redmond Red and Cyanine 5. In addition, with an
absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable
of effective performance in a wide range of colored FRET probes.
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
| Specifications |
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| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Freezer storage, -10 to -30°C, dry |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions |
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| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm |
|---|
| 10-5925-02 | 0.25 g | .25grams | 2.55 | 71.67 | 43 | 26.88 | 19.55 | 14.33 | 3.58 |
| 10-5925-90 | 100 µmol | .098grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5925-95 | 50 µmol | .049grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions |
|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm |
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| 10-5925-02 | 0.25 g | .25grams | 3.81 | 0.07 | 69.8 | 43.63 | 31.73 | 4.36 |
| 10-5925-90 | 100 µmol | .098grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
| 10-5925-95 | 50 µmol | .049grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200