| F-DMT-dC-CE Phosphoramidite |
Catalog Number: 10-1410-xx
Description: F-DMT-dC-CE Phosphoramidite
5'-Fluorous-dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C56H55F17N5O8P |
M.W.: 1280.02 |
F.W.: 289.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Normal |
| Please Note: Products for Fluorous Affinity Purification of Oligonucleotides: Patent pending, Berry & Associates, Inc. The use of these products is licensed under U.S. Patents 6,673,539, 6,156,896; 5,859,247; and 5,777,121 and one or more pending patents owned or controlled by Fluorous Technologies, Inc. |
FLUORO-PAK™ PURIFICATION
The fluorous affinity purification of oligonucleotides is a quick and simple affinity-based method for the purification of oligonucleotides that relies on the strong interaction of fluorous-tagged oligonucleotides (made with fluorous-tagged phosphoramidites) with the fluorinated adsorbent present in Fluoro-Pak™ columns. Fluorous affinity purification is operationally similar to DMT-on purification using a reverse-phase (RP) adsorbent, e.g., RP cartridge purification, except that it involves a much stronger affinity interaction and is thus able to afford higher selectivities and recoveries, even with long oligos.
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