| GAC Trimer Phosphoramidite |
Catalog Number: 13-1201-xx
Description: GAC Trimer Phosphoramidite
5'-Dimethoxytrityl-N2-isobutyryl-2'-deoxyGuanosine-3'->5'-o-chlorophenyl-
phosphoryl-N6-benzoyl-2'-deoxyAdenosine-3'->5'-o-chlorophenyl-phosphoryl-N4-
benzoyl-2'-deoxyCytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C89H92Cl2N15O21P3 |
M.W.: 1869.5 |
|
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling:15 minute coupling time recommended. |
| Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
EXAMPLE OF PREPARATION OF TRIMER MIXTURE
Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer from the right column. Dissolve the trimer mix in dichloromethane (highest grade possible; acid-free). Evaporate to dryness to produce a homogenous mixture of all 20 trimers.
EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER
Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.
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