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Oligo Synthesis

Oligo Synthesis : Purification

Prices quoted are for single packs only.

For multiples of the same product please request a quote.

Some of Glen's products are hazardous and may be subject to additional shipping charges.

Full product information is available on Glen Research's web site.

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Research

Catalogue No.DescriptionPack SizePriceQty
61-5002-05Glen Gel-Pak™ DNA/RNA desalting columns0.2ml capacity (pack of 5) £24.00£22.08Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
61-5002-50Glen Gel-Pak™ DNA/RNA desalting columns0.2ml capacity (pack of 50) £240.00£220.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
61-5010-05Glen Gel-Pak™ DNA/RNA desalting columns1.0ml capacity (pack of 5) £28.00£25.76Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
61-5010-50Glen Gel-Pak™ DNA/RNA desalting columns1.0ml capacity (pack of 50) £280.00£257.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
61-5025-05Glen Gel-Pak™ DNA/RNA desalting columns2.5ml capacity (pack of 5) £36.00£33.12Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
61-5025-25Glen Gel-Pak™ DNA/RNA desalting columns2.5ml capacity (pack of 25) £180.00£165.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Research

Catalog Number: 61-5002-xx

Description: Glen Gel-Pak™ 0.2 Desalting Column





Diluent: n/a

Technical Bulletin
Storage: Controlled room temperature or lower, dry
Stability in Solution:
IMPORTANT: REMOVE RED CAP FIRST, THEN REMOVE BOTTOM WHITE CAP.

 

Fig 1

GLEN GEL-PAK™ DESALTING

The principle of the Glen Research gel filtration column, Glen Gel-Pak™, is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through the resin of the column.

Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.

    There are many benefits to Glen Gel-Pak columns:
  • Versatility:
    • Ability to directly desalt oligonucleotides deprotected in either 30% ammonium hydroxide OR 50:50 ammonium hydroxide/40% aqueous methylamine (AMA)
    • Easily exchange buffers
    • Simple clean-up of labelling reactions
    • Mild method for purification from salts and solvents such as DMSO and DMF
  • Capacity:
    • Multiple column sizes (0.2 mL, 1.0 mL and 2.5 mL) are available to match synthesis scale
    • Ability to efficiently desalt short and long oligos at different scales using the same protocol
    • Suitable for oligos >10mer in length

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Research

Glen Report 22.2: New Products - Glen Gel-Pak(TM) DNA/RNA Desalting and 1000 Glen UnySupport(TM) Frits

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Gel-Pak™ DNA/RNA desalting columns

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
61-5002-05 Pk/5 1grams N/A N/A N/A N/A N/A N/A N/A
61-5002-50 Pk/50 1grams N/A N/A N/A N/A N/A N/A N/A
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
61-5002-05 Pk/5 1grams N/A N/A N/A N/A N/A
61-5002-50 Pk/50 1grams N/A N/A N/A N/A N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
61-5002-05 Pk/5 1grams N/A N/A N/A N/A
61-5002-50 Pk/50 1grams N/A N/A N/A N/A

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200