Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

p-Nitrophenyl phosphate (pNPP) substrate

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-2001-100p-Nitrophenyl phosphate (pNPP) substrate 5 mg 100 tablets £133.00 Quantity Add to Order
MC-09-2001-24p-Nitrophenyl phosphate (pNPP) substrate 5 mg 24 tablets / blisterpack £47.00 Quantity Add to Order
MC-09-2020-100p-Nitrophenyl phosphate (pNPP) substrate 20 mg 100 tablets £185.00 Quantity Add to Order
MC-09-2020-24p-Nitrophenyl phosphate (pNPP) substrate 20 mg 24 tablets / blisterpack £50.00 Quantity Add to Order

p-Nitrophenyl phosphate (pNPP) substrate

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays

Medicago AB

Product description

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3 M NaOH.

The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5 mg or 20 mg of substrate.

Features                      

  • Formulated from analytical grade reagents
  • Specifically developed for immunoassays
  • Chromogenic substrate                     
  • Reproducibility from lot to lot
  • Exactly pre-weighed tablets

 

Directions for use

Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use.

 

Shipping and storage

The pNPP substrate is shipped at room temperature. Store the tablets in a dark place below -18°C. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets

Weight: 5 mg and 20 mg

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

p-Nitrophenyl phosphate (pNPP) substrate

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays

Medicago AB

Applications                     

  • Immunoassays
  • Chromogenic substrate for phosphatase assays
  • Histochemistry procedures
  • Recommended for ELISA procedures

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200