Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Saline Sodium Citrate (SSC 2x)

Medicago’s SSC buffer is specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. SSC buffer is an acid salt buffer solution and is used as a buffering agent to control acidity

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-9501-100Saline Sodium Citrate (SSC 2x) pH 7.0, 100 ml 100 tablets £46.00 Quantity Add to Order
MC-12-9195-5Saline sodium citrate buffer (SSC) 20x pH 7.0, 1000 ml 5 pouches £72.00 Quantity Add to Order
MC-12-9196-5Saline sodium citrate buffer (SSC) 2x pH 7.0, 1000 ml 5 pouches £36.00 Quantity Add to Order

Saline Sodium Citrate (SSC 2x)

Medicago’s SSC buffer is specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. SSC buffer is an acid salt buffer solution and is used as a buffering agent to control acidity

Medicago AB

Product description

Medicago’s SSC buffer is specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. SSC  buffer is an acid salt buffer solution and is used as a buffering agent to control acidity. The buffer is supplied in two sizes giving 100 ml or 1000 ml of 0.30 M sodium citrate, 0.030 M sodium chloride buffer or 1000 ml of 3.0 M sodium citrate, 0.30 M sodium chloride buffer with pH 7.0 at 25°C when one tablet or the contents of one pouch is dissolved in deionized water.

Features

  • Formulated from analytical grade chemicals
  • Two stock solutions: 2x and 20x
  • Buffering agent to control acidity
  • Choice of convenient tablets or powder in pouches
  • Eliminates tedious pH adjustments         

 

 

Directions for use

Empty one pouch or deposit one tablet of the SSC buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml or 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 100 ml or 1000 ml, stir until full dissolution and the buffer stock solution is ready to use.

 

Shipping and storage

SSC buffer is shipped at room temperature. Store the tablets and pouches in a dry place at room temperature. Shelf life is three years after production date. Stock solutions can be stored at room temperature or at 2°C to 8°C for two months.

 

Specifications

Chemicals: Analytical grade 

Format: Exactly pre-weighed powder mix and tablets

Composition 20x: 3.0 M sodium citrate, 0.30 NaCl buffer

Composition 2x: 0.30 M sodium citrate, 0.030 M NaCl buffer

Volume: 100 ml and 1000 ml

pH: 7.0 ± 0.05 at 25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Saline Sodium Citrate (SSC 2x)

Medicago’s SSC buffer is specifically developed for Northern and Southern transfer protocols and nucleic acid preparations. SSC buffer is an acid salt buffer solution and is used as a buffering agent to control acidity

Medicago AB

Applications

  • Nucleic acid hybridization
  • Blot transfer procedures
  • Nucleic acid preparation

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200