Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Tris Buffered Saline with Tween® 20 (TBS-T)

TBS Tween™20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-09-7510-10Tris Buffered Saline with Tween® 20 (TBS-T) pH 7.6, 500 ml 10 tablets £30.00 Quantity Add to Order
MC-09-7510-100Tris Buffered Saline with Tween® 20 (TBS-T) pH 7.6, 500 ml 100 tablets £148.00 Quantity Add to Order
MC-09-7510-5Tris Buffered Saline with Tween® 20 (TBS-T) pH 7.6, 500 ml 5 tablet sample (1 per customer) £0.00 Quantity Add to Order

Tris Buffered Saline with Tween® 20 (TBS-T)

TBS Tween™20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies.

Medicago AB

Product description

TBS Tween™20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies.

Medicago’s TBS-T buffer is supplied in exactly pre-weighed tablets, each tablet giving 500 ml of 0.15 M NaCl, 0.05% TweenTM 20, 0.050 M Tris-HCl buffer solution with pH 7.6 at 25°C.

 

Features

  • Formulated from analytical grade chemicals  
  • TBS buffer containing non-ionic detergent
  • Exactly pre-weighed tablets
  • Eliminates variables in laboratory work flow

 

 

Directions for use

Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 200 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 500 ml, stir until full dissolution and the buffer is ready to use.

 

Shipping and storage

TBS-T buffer is shipped at room temperature. Store the tablets in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets

Composition: 0.14 M NaCl, 0.050 M HCl, 0.05 % Tween™ 20

Volume: 500 ml

pH: 7.6 ± 0.05 at 25°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Tris Buffered Saline with Tween® 20 (TBS-T)

TBS Tween™20 is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies.

Medicago AB

Applications

  • Blocking buffer for plate based assays
  • Wash buffer in immunoassays, for example ELISA and Western blotting

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200