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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Artocarpus integrifolia lectin (Jacalin)

Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure with a weight of 66 kDa

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0133-10Artocarpus integrifolia lectin (Jacalin) 10 mg £68.00 Quantity Add to Order
MC-05-0133-100Artocarpus integrifolia lectin (Jacalin) 100 mg £483.00 Quantity Add to Order

Artocarpus integrifolia lectin (Jacalin)

Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure with a weight of 66 kDa

Medicago AB

Product Description

 Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure (Figure 1) with a weight of 66 kDa (2).

Lectins are, due to their specific binding to carbohydrate structures on the cell surface or elsewhere useful in haematology, immunology or as specific markers for membrane glycoprotein structures (2). Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. The lectin is blood group non-specific after neuraminidase treatment and agglutinates human erythrocytes at a concentration of ≥ 7,8 µg/ml.

A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the a-chain. The relative affinities of the lectin for galactose derivatives, as well as the structural basis of its T-antigen specificity, are explained by its protein structure (2).

Medicago’s Jacalin is supplied as a white lyophilized powder from a buffer containing 50 mM NH4HCO3, 10 µM CaCl2, no preservatives are added. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 12 kDa and 16 kDa. The lectin concentration is determined by amino acid analysis, minimum 80%. The material is made from one single batch; it is homogenous and is not derived from any material of animal origin. It is available in vials containing 100 mg or 10 mg lyophilized powder and is to be used for laboratory work only.


Features 

  • Ultrapure quality
  • Galactose-binding lectin
  • Specific for a tumour-associated T-antigenic disaccharide
  • Agglutinates human erythrocytes at a concentration of ≥ 7.8 µg/ml
  • Lyophilized powder

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Artocarpus integrifolia lectin (Jacalin)

Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure with a weight of 66 kDa

Medicago AB

 

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

(2) Sankaranarayanan R., Sekar K., Banerjee R., Sharma V., Surolia A., Vijayan M, (1996)  A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a b-prism fold. Nature Structural Biology 3, 596–603.

(3) Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose. Bourne, Y., Astoul, C.H., Zamboni, V., Peumans, W.J., Menu-Bouaouiche, L., Van Damme, E.J., Barre, A., Rouge, P. (2002) Biochem.J. 364: 173–180.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Artocarpus integrifolia lectin (Jacalin)

Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure with a weight of 66 kDa

Medicago AB

Specifications

Appearance: White to light-yellow lyophilized powder                              

Source: Jackfruit seeds                                                                                                                           

Activity: Agglutinates human erythrocytes at a concentration of ≥ 7,8 μg/ml. Blood group non-specific after neuraminidase treatment.

Microorganisms: < 100 CFU/g                                                              

Protein: > 80 %                                                                                                                            

Identity: SDS-PAGE, two homogeneous bands at 12 kDa and 16 kDa.                              

Shelf life: > Five years when stored at -20°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Artocarpus integrifolia lectin (Jacalin)

Artocarpus integrifolia lectin (Jacalin) is isolated from jackfruit seeds and purified by affinity chromatography. The lectin belongs to the family of galactose-binding lectins (1) and it has a tetrameric two-chain structure with a weight of 66 kDa

Medicago AB

Applications

  • Isolation of IgA from human serum
  • Isolation of human plasma glycoproteins
  • AIDS research

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200