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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Phaseolus vulgaris lectin P (PHA-P)

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides

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Catalogue No.DescriptionPack SizePriceQty
MC-05-0115-10Phaseolus vulgaris lectin P (PHA-P) 10 mg £87.00 Quantity Add to Order
MC-05-0115-5Phaseolus vulgaris lectin P (PHA-P) 5 mg £50.00 Quantity Add to Order

Phaseolus vulgaris lectin P (PHA-P)

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides

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Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides (1). PHA-P is not blood group specific.

Medicago’s PHA-P lectin is supplied as a white to light pink lyophilized powder from a buffer containing 50 mM NH4HCO3. The purity of the lectin is determined by SDS-PAGE generating one single band at ???. PHA-P is available in vials containing 10 mg or 5 mg lyophilized powder and the product is to be used for laboratory work only.

 Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

 

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phaseolus vulgaris lectin P (PHA-P)

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides

Medicago AB

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phaseolus vulgaris lectin P (PHA-P)

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides

Medicago AB

Specifications

Appearance: White to light pink lyophilized powder or flocculate               

Source: Red kidney beans

Molecular weight: 115 kDa

Sugar specificity: Complex oligosaccharides

Activity: Agglutinates human erythrocytes

Microorganisms: < 100 CFU/g

Protein content: > 75 %, OD280

Identity: SDS-PAGE, one major band

Shelf life: > Three years when stored at -20°

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Phaseolus vulgaris lectin P (PHA-P)

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P) is isolated from red kidney beans and purified by affinity chromatography. The lectin consists of the two subunits lectins PHA-E (erythroagglutinin) and PHA-L (leucoagglutinin) (1) thus PHA-P is the form of PHA before separation of erythroagglutinin and leucoagglutinin. PHA-P has a molecular weight of 115 kDa and specificity for complex oligosaccharides

Medicago AB

Applications

  • Lateral flow erythrocyte separation

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200