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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

5’- DBCO-TEG Phosphoramidite

5’- DBCO-TEG Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1941-025’-DBCO-TEG Phosphoramidite0.25g £620.00 Quantity Add to Order
10-1941-905’-DBCO-TEG Phosphoramidite100 µmole £184.00 Quantity Add to Order
10-1941-955’-DBCO-TEG Phosphoramidite50 µmole £100.00 Quantity Add to Order

5’- DBCO-TEG Phosphoramidite

5’- DBCO-TEG Phosphoramidite

Glen Research

5Õ-DBCO-TEG Phosphoramidite

 

 

Structure

Catalog Number: 10-1941-xx

Description: 5Õ-DBCO-TEG Phosphoramidite

10-(6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-capramido-N-ethyl)-
O-triethyleneglycol-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-
phosphoramidite
Formula: C38H53N4O7P M.W.: 708.82 F.W.: 570.57

Diluent: Anhydrous Acetonitrile
Coupling: 10 minute coupling time recommended.
Deprotection: Compatible with 30% ammonium hydroxide for 2 hours at 65 °C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results.
Storage: Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

COPPER-FREE CLICK CHEMISTRY

At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:

  • Simple to use
  • Stable in solution on the synthesizer
  • Stable to ammonium hydroxide and AMA
  • Excellent click performance in 17 hours or less at room temperature

From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5’-DBCO-TEG Phosphoramidite for preparing oligos with a 5’-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5’- DBCO-TEG Phosphoramidite

5’- DBCO-TEG Phosphoramidite

Glen Research

5’- DBCO-TEG Phosphoramidite

5’- DBCO-TEG Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1941-95 50µmoles .035grams .5 3.33 2 1.25 .91 .67 .17
10-1941-90 100µmoles .071grams 1 20 12 7.5 5.45 4 1
10-1941-02 0.25grams .25grams 3.53 104.33 62.6 39.13 28.45 20.87 5.22
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1941-95 50µmoles .035grams .75 .07 8.6 5.38 3.91 .54  
10-1941-90 100µmoles .071grams 1.5 .07 23.6 14.75 10.73 1.48  
10-1941-02 0.25grams .25grams 5.26 .07 98.8 61.75 44.91 6.18  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1941-95 50µmoles .035grams .75 .07 10.2 6.38 4.64    
10-1941-90 100µmoles .071grams 1.5 .07 25.2 15.75 11.45    
10-1941-02 0.25grams .25grams 5.26 .07 100.4 62.75 45.64  

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200