Catalog Number: 10-1941-xx
Description: 5Õ-DBCO-TEG Phosphoramidite
Diluent: Anhydrous Acetonitrile
|Coupling: 10 minute coupling time recommended.
|Deprotection: Compatible with 30% ammonium hydroxide for 2 hours at 65 °C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results.
|Storage: Storage: Freezer storage, -10 to -30°C, dry
|Stability in Solution: 1-2 days
COPPER-FREE CLICK CHEMISTRY
At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
- Simple to use
- Stable in solution on the synthesizer
- Stable to ammonium hydroxide and AMA
- Excellent click performance in 17 hours or less at room temperature
From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5’-DBCO-TEG Phosphoramidite for preparing oligos with a 5’-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.
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