Cambio - Excellence in Molecular Biology

Molecular Cloning Kits

Molecular Cloning Kits: Cloning Kits

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MP5105MaxPlax™ Lambda Packaging Extract5 Extracts £182.00£182.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
MP5110MaxPlax™ Lambda Packaging Extract10 Extracts £281.00£281.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
MP5120MaxPlax™ Lambda Packaging Extract20 Extracts £448.00£448.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

Maximized packaging efficiency of lambda, cosmid, or fosmid DNA for the construction of large insert genomic libraries.

  • Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.
  • Packaging efficiencies of up to 3 × 109 pfu/µg DNA.
  • Simplified protocol does not require premixing of different extract components before use.

stating actual packaging efficiency (pfu/µg DNA) for each respective lot of MaxPlax™ Extract is provided.

MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.

Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 × 109 pfu/µg of Control Ligated Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.

Table 1. Packaging of methylated and unmethylated DNA using MaxPlax™ Lambda Packaging Extracts.
Cosmid Insert Pfu/µg DNA
Unmethylated DNA 3.5 x 107
Methylated DNA 3.9 x 107

References

  1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
  2. Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
  3. Fiandt, M. (2000) Epicentre Forum 7(3), 14

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: MaxPlax™ Lambda Packaging Extracts 

MaxPlax™ Lambda Packaging Extracts Protocol

(catalogue number MP5105 / MP5110 / MP5120 

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

References 

  1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
  2. Gunther, E.G. et al. (1993) Nucl. Acids Res. 21, 3903.
  3. Fiandt, M. (2000) EPICENTRE Forum 7 (3), 14.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

Epicentre® Forum Get your Epicentre® Forum - hot off the press!
Click here for direct link to Forum listings

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MaxPlax™ Lambda Packaging Extract

MaxPlax Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.

BioSearch Tech (Lucigen/Epicentre)

 Applications

  •   Packaging of lambda, cosmid, or fosmid DNA for construction of genomic libraries.

Benefits

  • Packaging efficiencies of up to 3 x 109 pfu/µg DNA.
  • MaxPlax Extracts are devoid of all known restriction activities. Packages highly methylated DNA as efficiently as unmethylated DNA.3
  • No premixing of different extract components required before use.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200