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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
EN510250Exonuclease VII, E. coli 250U £189.00£189.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
EX4425KExonuclease III, E. coli200U/µl 25,000U £172.00£172.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

Exonuclease III digests duplex DNA in a 3'Æ5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results. 

Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios,3,4 each template must be optimised using sample digestions to achieve the desired excision rate. 

Exonuclease III is not active on 3'-protruding ends of 4 bases or more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3'-DNA phosphatase, and apurinic DNA endonuclease activities. 

Unit Definition

One unit catalyses the release of 1nmole of acid-soluble nucleotides from double-stranded calf thymus DNA in 30 minutes at 37°C in 33mM Tris-acetate, pH 7.8, 66mM potassium acetate, 10mM magnesium acetate, and 0.5mM DTT. 

Storage Buffer

50% glycerol containing 50 mM Tris-HCl, pH 7.5, 0.1M NaCl, 0.1mM EDTA, 1mM DTT, and 0.1% Triton® X-100. 

Exonuclease III 10X Reaction Buffer

330mM Tris-acetate, pH 7.8, 660mM potassium acetate, 100mM magnesium acetate, and 5mM DTT. 

Quality Control

Exonuclease III is free of detectable exogenous RNase, endonuclease, and single-stranded exonuclease activities.






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Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Exonuclease III, E. coli and Exonuclease VII

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 

Exonuclease III Protocol

(catalogue number EX4405K / EX4425K)

Please note: all protocols off site are the responsibility of the products supplier

Exonuclease VII Protocol

(catalogue number EN510100 / EN510250)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

References

Exonuclease III

  1. Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York.
  2. Vandeyar, M.A. (1988) Gene 65, 129.
  3. Luckow, B. et al. (1987) Nucleic Acids Res. 15, 417.
  4. Richardson, C.C. et al. (1964) J. Biol. Chem. 239, 251.
  5. Weiss, B. (1976) J. Biol. Chem. 251, 1896.
  6. Rogers, S.G. and Weiss, B. (1980) Meth. Enzymol. 65, 201.
  7. Guo, L.H. and Wu, R. (1982) Nucleic Acids Res. 10, 2065.

Exonuclease VII

  1. Li, H. et al. (1991) Nucleic Acids Res. 19, 3139.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exonuclease III, E. coli and Exonuclease VII

Exonuclease III digests duplex DNA in a 3' → 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Production of intermediates for site-directed mutagenesis.1-3
  • Production of strand-specific radiolabeled probes.4

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200