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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

S1 Nuclease (Aspergillus oryzae)

S1 Nuclease catalyses the release of 5'-phosphoryl mono- and oligonucleotides from single stranded nucleic acids

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Catalogue No.DescriptionPack SizePriceQty
PM-1210-02S1 Nuclease50kU £550.00 Quantity Add to Order
PM-1210-10S1 Nuclease10kU £250.00 Quantity Add to Order

S1 Nuclease (Aspergillus oryzae)

S1 Nuclease catalyses the release of 5'-phosphoryl mono- and oligonucleotides from single stranded nucleic acids

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Source

Aspergillus oryzae

Applications

  • Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5'-mononucleotides (1)
  • Cleaves both single-stranded DNA and RNA, with stronger DNAse activity
  • Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration
  • Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps (2)
  • Relaxes/linearizes supercoiled plasmids
  • Removes protruding single-stranded ends
  • Can generate double-stranded DNA breaks in response to DNA nicks abasic sites
  • Used for S1 mapping of nucleic acids
  • Requires Zn² ions for activity
  • The enzyme is active up to 65°C

Unit Definition

One unit is the amount of enzyme required to convert 1 µg of denatured calf thymus DNA to an acid soluble form in 1 minute at 37°C.

Storage Buffer

20 mM Sodium Acetate (pH 4.6)
50 mM NaCl
1 mM ZnCl2
50% (v/v) glycerol

Quality Control

All preparations are assayed for contaminating exonuclease and double-stranded DNase activities.

Storage Conditions

Store at -20°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

S1 Nuclease (Aspergillus oryzae)

S1 Nuclease catalyses the release of 5'-phosphoryl mono- and oligonucleotides from single stranded nucleic acids

Cambio

Download here

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

S1 Nuclease (Aspergillus oryzae)

S1 Nuclease catalyses the release of 5'-phosphoryl mono- and oligonucleotides from single stranded nucleic acids

Cambio

(1) Berg, A.J. et al. (1977) Cell 12, 721 (2) Sambrook, J. et al. (1989) Molecular cloning: A laboratory Manual, second edition, pp. 5.78-5.79, Cold Spring Harbor, New York

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200