Transfection-EZ Biosystems: Broad Range Transfection Reagents
We offer a range of EZ Biosystems' general purpose transfection reagents for plasmid, siRNA, co-transfections, in vivo work and CRISPR. These reagents are cost-effective and have been specially formulated for maximum transfection efficiency of a wide range of different cell types with minimal toxicity.
Avalanche® transfection reagents have the broad spectrum for
the transfection of large plasmid, mRNA, siRNA, and/or other type of
nucleic acids. This feature makes Avalanche® transfection reagents the
best option for co-transfection of different type and/or size of nucleic
acids. The following are the reasons for the broad spectrum:
The
key components of Avalanche® transfection reagents are mixtures of
cationic lipids and cationic polymers, which contain all three types of
amines: primary, secondary, and tertiary amines. The three types of
amines have different positive charge strength, hence have different
capabilities to combine with negative charged anions.
As anions, different types and sizes of nucleic acids have different densities and quantities of negative charges.
With
optimized ratios of different cationic lipids and polymers, hence
optimized ratios of the three different type of amines, together with
optimized protocols, our Avalanche® mixtures have the optimized strength
of positive charges that ensure them to efficiently combine with
different type of nucleic acids of a variety of negative charge
densities and quantities, and compact them into the right sizes of
nanoparticles with the right densities of positive charge (zeta
potential) on their surface. The optimized size and the right strength
of zeta potential of the nanoparticles are the keys for the
nanoparticles to interact efficiently with negatively charged cell
surface, and trigger endocytosis process.
Avalanche-Omni Transfection Reagent
Avalanche®-Omni Transfection Reagent is an exceptionally powerful and versatile next-generation DNA and siRNA transfection reagent for day-to-day experiments. As a new, proprietary formulation of lipids and polymers, Avalanche®-Omni Transfection Reagent has been shown to effectively transfect the broadest spectrum of adherent and suspension cells. No other transfection reagents can match the efficiency, spectrum, convenience, and gentleness of Avalanche®-Omni Transfection Reagent for transfection of primary cells and other difficult-to-transfect ..
EZ Biosystems
Description
Avalanche®-Omni Transfection Reagent is an exceptionally powerful and versatile next-generation DNA and siRNA transfection reagent for day-to-day experiments. As a new, proprietary formulation of lipids and polymers, Avalanche®-Omni Transfection Reagent has been shown to effectively transfect the broadest spectrum of adherent and suspension cells. No other transfection reagents can match the efficiency, spectrum, convenience, and gentleness of Avalanche®-Omni Transfection Reagent for transfection of primary cells and other difficult-to-transfect cells. Avalanche®-Omni Transfection Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.
Features:
-
Exceptional transfection efficiency in the broadest range of cell types including cell lines of stem cell origin, suspension cells, and primary cells with less than half the amount required by competitors’ products.
-
Extremely gentle to cells: The chemical features of our proprietary formula and the least amount needed for transfection ensure its lowest toxicity.
-
Economical: High efficiency means less amount of nucleic acid & reagent is needed. 1.5 ml of Avalanche™-Omni Reagent is sufficient for more than 3,500 transfections in 24-well plates.
-
Superior performance for co-transfection of siRNA and plasmid DNA
-
Proven efficacy in the presence of serum: eliminates the need to change media following transfection
-
Animal free
-
Reliable performance for high-throughput applications
-
The best choice for establishing stable cell lines
-
Simplest protocol: simply mix with nucleic acid and add to cell culture.
Data:
 |
| Figure 1. Avalanche®-Omni Transfection Reagent revealed highest efficiency and optimal balance of potency & low cytotoxicity. NIH 3T3 cells were transfected with enhanced green fluorescent protein (eGFP) expressing vector by using our Avalanche®-Omni Transfection Reagent and several most popular commercial transfection reagents. The transfection result was evaluated by FACS using three criteria: the percent of GFP positive cells (transfection efficiency), the percent of dead cells (toxicity, 7AAD positive cells), and mean fluorescence intensity (MFI, expression level of GFP in cells).(A) Pictures of GFP expression in cells transfected with Avalanche®-Omni and other commercial transfection reagents. (B) FACS data showed the diffeences on transfection efficiency between Avalanche®-Omni and other commercial transfection reagents. (C) Quantitative data of (B) FACS analysis: Avalanche®-Omni showed the highest transfection efficiency and the optimal balance of potent & low cytotoxicity. GFP expression level in cells are high as well in Avalanche®-Omni Transfection Reagent group. |
| |
| Avalanche®-Omni Transfection Reagent is able to achieve more than 90% knockdown of endogenous gene expression in a variety of cell lines and primary cells. |
 |
| Figure 2. Avalanche®-Omni Transfection Reagent performed more than 90% knockdown of endogenous gene expression. (A). ROCK1 mRNA levels were quantified using qPCR in Hela cells transfected with Target-specific siRNA duplexes (10nM) for human ROCK1 gene or non-silencing siRNA by using Avalanche®-Omni Transfection Reagent. Data were normalized against the 18S rRNA signal. Control Samples were either mock-transected or untreated. Values are normalized to untreated sample. Data are means±SD (n=3). (B) Actin stress fibers were stained with FITC-labelled phalloidin on the cells in (A) cultured on fibronectin-coated micropattern. Confocal Microscope revealed that the one transfected with ROCK1 siRNA (right) showed disrupted stress fiber pattern as compared to the one transfected with non-silencing siRNA (left). |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Protocols
Download protocol here
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
References
Avalanche®-Omni Transfection Reagent is an exceptionally powerful and versatile next-generation DNA and siRNA transfection reagent for day-to-day experiments. As a new, proprietary formulation of lipids and polymers, Avalanche®-Omni Transfection Reagent has been shown to effectively transfect the broadest spectrum of adherent and suspension cells. No other transfection reagents can match the efficiency, spectrum, convenience, and gentleness of Avalanche®-Omni Transfection Reagent for transfection of primary cells and other difficult-to-transfect cells. Avalanche®-Omni Transfection Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.
Features:
-
Exceptional transfection efficiency in the broadest range of cell types including cell lines of stem cell origin, suspension cells, and primary cells with less than half the amount required by competitors’ products.
-
Extremely gentle to cells: The chemical features of our proprietary formula and the least amount needed for transfection ensure its lowest toxicity.
-
Economical: High efficiency means less amount of nucleic acid & reagent is needed. 1.5 ml of Avalanche™-Omni Reagent is sufficient for more than 3,500 transfections in 24-well plates.
-
Superior performance for co-transfection of siRNA and plasmid DNA
-
Proven efficacy in the presence of serum: eliminates the need to change media following transfection
-
Animal free
-
Reliable performance for high-throughput applications
-
The best choice for establishing stable cell lines
-
Simplest protocol: simply mix with nucleic acid and add to cell culture
Data:
 |
| Figure 1. Avalanche®-Omni Transfection Reagent revealed highest efficiency and optimal balance of potency & low cytotoxicity. NIH 3T3 cells were transfected with enhanced green fluorescent protein (eGFP) expressing vector by using our Avalanche®-Omni Transfection Reagent and several most popular commercial transfection reagents. The transfection result was evaluated by FACS using three criteria: the percent of GFP positive cells (transfection efficiency), the percent of dead cells (toxicity, 7AAD positive cells), and mean fluorescence intensity (MFI, expression level of GFP in cells).(A) Pictures of GFP expression in cells transfected with Avalanche®-Omni and other commercial transfection reagents. (B) FACS data showed the diffeences on transfection efficiency between Avalanche®-Omni and other commercial transfection reagents. (C) Quantitative data of (B) FACS analysis: Avalanche®-Omni showed the highest transfection efficiency and the optimal balance of potent & low cytotoxicity. GFP expression level in cells are high as well in Avalanche®-Omni Transfection Reagent group. |
| |
| Avalanche®-Omni Transfection Reagent is able to achieve more than 90% knockdown of endogenous gene expression in a variety of cell lines and primary cells. |
 |
| Figure 2. Avalanche®-Omni Transfection Reagent performed more than 90% knockdown of endogenous gene expression. (A). ROCK1 mRNA levels were quantified using qPCR in Hela cells transfected with Target-specific siRNA duplexes (10nM) for human ROCK1 gene or non-silencing siRNA by using Avalanche®-Omni Transfection Reagent. Data were normalized against the 18S rRNA signal. Control Samples were either mock-transected or untreated. Values are normalized to untreated sample. Data are means±SD (n=3). (B) Actin stress fibers were stained with FITC-labelled phalloidin on the cells in (A) cultured on fibronectin-coated micropattern. Confocal Microscope revealed that the one transfected with ROCK1 siRNA (right) showed disrupted stress fiber pattern as compared to the one transfected with non-silencing siRNA (left). |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200