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Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated. 

For primary cells click HERE

To learn why you should use Avalanche® Cell Type-Specific Transfection reagents, click HERE; To learn how EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents, click HERE

UO-31 Cell Avalanche® Transfection Reagent (human kidney carcinoma cell)

UO-31 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on UO-31 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for UO-31 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of UO-31 Cell Avalanche® Transfection Reagent for transfection on UO-31 cells.

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
EZT-UO31-1-05UO-31 Cell Transfection Reagent (human kidney carcinoma cell)0.5ml £399.00 Quantity Add to Order
EZT-UO31-1-15UO-31 Cell Transfection Reagent (human kidney carcinoma cell)1.5ml £699.00 Quantity Add to Order

UO-31 Cell Avalanche® Transfection Reagent (human kidney carcinoma cell)

UO-31 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on UO-31 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for UO-31 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of UO-31 Cell Avalanche® Transfection Reagent for transfection on UO-31 cells.

EZ Biosystems

Cell Line Information:
Cell Line Name: UO-31, UO.31, UO31
Patient's Sex: F
Prior Treatment: No
Tissue Type: Kidney
Histology: Renal cell carcinoma-vpd; epithelium
p53 Status: 1
ECAD Methylation: 60
MDR Function: 59
Institution: National Cancer Institute
Contributor: Linehan, WM
Uo-31 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
UO-31 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on UO-31 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for UO-31 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of UO-31 Cell Avalanche® Transfection Reagent for transfection on UO-31 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

  • Specifically optimized to deliver nucleic acids into UO-31 cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Active in serum-containing medium
  • Suitable for Reverse Transfection  
  • Compatible with transfection in any plate format
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
 
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on UO-31 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for UO-31 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this UO-31 Cell Avalanche® Transfection Reagent.
 

For Other Cells:
UO-31 Cell Avalanche® Transfection Reagent (human kidney carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A498 Cell  
ACHN Cell  
RXF393 Cell  
SN12C Cell  
TK10 Cell  
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200