Cyanine 5 CPG
1-[3-(4-monomethoxytrityloxy)propyl]-3,3,3',3'-tetramethylindodicarbocyanine chloride-1'-propyl-3-O-succinoyl-long chain alkylamino-CPG
Coupling: Monomers that allow for UltraMILD deprotection are recommended. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: As required by nucleobases. If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at Room Temperature in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields. However, the oligonucleotide will require RP HPLC purification.
Storage: Storage: Freezer storage, -10 to -30°C, dry
Non-Catalog Item. Please contact customer service for pricing and availability.
If you cannot find the answer to your problem below then please
contact us or telephone 01954 210 200