Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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For multiples of the same product please request a quote.

Some of Glen's products are hazardous and may be subject to additional shipping charges.

Full product information is available on Glen Research's web site.

Glen UnySupport™ FC

Glen UnySupport™ FC N-Methyl-succinimido[3,4-b]-7-oxabicyclo[2.2.1]heptane-6-(4,4'-dimethoxytrityloxy)-5-diglycoloyl-long chain alkylamino CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
22-5041-01Glen UnySupport™ FC0.1 g POA Quantity Add to Order
22-5041-02Glen UnySupport™ FC0.25 g POA Quantity Add to Order
22-5041-10Glen UnySupport™ FC1.0 g POA Quantity Add to Order
22-5141-13Glen UnySupport™ FC1 x 10 umole POA Quantity Add to Order
22-5141-41Glen UnySupport™ FCpack/4 1umole POA Quantity Add to Order
22-5141-42Glen UnySupport™ FCpack/4 0.2umole POA Quantity Add to Order
22-5141-45Glen UnySupport™ FCpack/4 40nmole POA Quantity Add to Order
22-5141-52Glen UnySupport™ FC AB 3900 formatpack/10 200nmole £80.00£73.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5141-55Glen UnySupport™ FC AB 3900 formatpack/10 40nmole £80.00£73.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5141-91Glen UnySupport™ FC 96 well formatpack/96 1umole £300.00£276.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5141-92Glen UnySupport™ FC 96 well formatpack/96 0.2umole £200.00£184.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5141-95Glen UnySupport™ FC 96 well formatpack/96 40nmole £200.00£184.00Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5241-14Glen UnySupport™ FC Expedite format1 x 15 µmole column (TWIST Format) £120.00£110.40Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5241-41Glen UnySupport™ FC Expedite formatpack/4 1umole £48.00£44.16Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5241-42Glen UnySupport™ FC Expedite formatpack/4 0.2umole £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
22-5241-45Glen UnySupport™ FC Expedite formatpack/4 40nmole £32.00£29.44Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Glen UnySupport™ FC

Glen UnySupport™ FC N-Methyl-succinimido[3,4-b]-7-oxabicyclo[2.2.1]heptane-6-(4,4'-dimethoxytrityloxy)-5-diglycoloyl-long chain alkylamino CPG

Glen Research

Glen UnySupport FC

 

The extended time required to cleave the succinate linkage of the original Glen UnySupport can be problematical, especially in high-throughput production of oligos, due to the outgassing of ammonia and/or methylamine. This reduction in concentration of gas can necessitate the evaporation of the cleavage solution and addition of fresh Ammonium Hydroxide:MethylAmine 1:1 (AMA) or ammonium hydroxide (NH4OH) to ensure complete deprotection and dephosphorylation of the product oligos. Using a diglycolate linkage in Glen UnySupport FC instead of the succinate in Glen UnySupport, a significant increase in the rate of cleavage has been achieved. The minimum cleavage times for both versions are as follows:

 

  AMA NH4OH
Glen UnySupport 10 min. 40 min.
Glen UnySupport FC 2 min. 5 min.

With the cleavage time of Glen UnySupport FC reduced to less than 5 minutes, there is minimal loss of volatile gas and, therefore, no need to evaporate the cleavage solution and replenish with fresh AMA or ammonium hydroxide solutions.

We offer Glen UnySupport FC attached to 1000Å CPG in a variety of formats suited to high throughput synthesis, as well as in bulk for more routine use.

 

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.

Deprotection:  Solution phase:  Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65°C or Ammonium Hydroxide for 8 hours at 55°C. For sensitive minor bases or dyes, Glen UnySupport may be eliminated with 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60°C.  Gas Phase:  Methylamine gas for 30 minutes at 65°C at 30 psi. Oligonucleotide is cleaved from the support in 2 minutes (AMA) or 5 minutes (NH4OH) at room temperature.

Storage: Storage: Storage: Controlled room temperature or lower, dry 

Please Note: This product is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..


Non-Catalog Item. Please contact customer service for pricing and availability. 
 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Glen UnySupport™ FC

Glen UnySupport™ FC N-Methyl-succinimido[3,4-b]-7-oxabicyclo[2.2.1]heptane-6-(4,4'-dimethoxytrityloxy)-5-diglycoloyl-long chain alkylamino CPG

Glen Research

Safety Data Sheet 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Glen UnySupport™ FC

Glen UnySupport™ FC N-Methyl-succinimido[3,4-b]-7-oxabicyclo[2.2.1]heptane-6-(4,4'-dimethoxytrityloxy)-5-diglycoloyl-long chain alkylamino CPG

Glen Research

Glen Report 25.28: Technical Brief - Glen UnySupport now Available with Fast Cleavage

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200