Cambio - Excellence in Molecular Biology

Transfection - Biontex

Transfection - Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

micro-Transfection Kit VI - microfection kit

Cell culture, transfection and high-resolution microscopy directly in the culture vessel

Biontex

Catalogue No.DescriptionPack SizePriceQty
K010-100micro-Transfection Kit VI100ul £325.00 Quantity Add to Order

micro-Transfection Kit VI - microfection kit

Cell culture, transfection and high-resolution microscopy directly in the culture vessel

Biontex

  • Simple transfection protocol (no optimization necessary)
  • DNA and siRNA transfection
  • Immediate live observation possible
  • Ideal optical properties for transmitted light and fluorescence microscopy
  • Cells can be fixed and dyed
  • Compatible with oil immersion

The μ-Transfection Kit VI is a unique combination of two elements tailored to each other in a highperformance kit permitting high-resolution microscopy of living transfected cells. The joint kit comprises μ-Slides VI from ibidi and the reagent METAFECTENE® μ, developed by Biontex especially for this purpose.

The kit enables cell transfection to be performed with both DNA and siRNA. The optimization procedure usually necessary in plasmid transfection is eliminated, while in siRNA transfection optimum knockdown is achieved with only three differing lipoplex volumes.

The ultra-simple protocol provides for treatment of a cell suspension with lipoplexes and transfer directly onto the μ-Slides. The resulting effects can be subsequently observed in the living cells in outstanding optical quality (live cell imaging). Examples of applications are tracking the expression and path of a fluorescence-tagged protein through the cell or monitoring the effect of knockdown of a gene.

High-resolution images of transfection of HepG2 cells with pCMV-GFP using μ-Transfection Kit VI (60× oil-immersion objective)

High-resolution images of transfection of HeLa cells with pCMV-GFP using μ-Transfection Kit VI (20× objective)

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

micro-Transfection Kit VI - microfection kit

Cell culture, transfection and high-resolution microscopy directly in the culture vessel

Biontex

µ-Transfection Kit VI 100 μl METAFECTENE® μ 10 ml 1 x PBS 15 Slides Transfection kit for microscopy

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

micro-Transfection Kit VI - microfection kit

Cell culture, transfection and high-resolution microscopy directly in the culture vessel

Biontex

Transfection and high-resolution microscopy of living mammalian cells (live cell imaging)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200