Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Reagents

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Thiazole Orange NHS Ester

Thiazole Orange NHS Ester

Glen Research

Catalogue No.DescriptionPack SizePriceQty
50-1970-23Thiazole Orange NHS Ester 5.4mg £48.00£45.60Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
50-1970-SPThiazole Orange NHS Ester 1.0g £4,616.00£4,385.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Thiazole Orange NHS Ester

Thiazole Orange NHS Ester

Glen Research

Thiazole Orange NHS Ester

N-(5-carboxypentyl)-2-[(1,4-dihydro-1- methylquinolin-4-ylidene)methyl]benzothiazol-3-ium chloride N-hydroxysuccinimide ester

Thiazole Orange NHS Ester

Product Specifications

Formula:
C28H28ClN3O4S
M.W.:
538.06
F.W.:
386.51

Description

Thiazole orange is an asymmetric cyanine dye whose fluorescence can be quite dependent on its local environment. When an oligonucleotide labeled with thiazole orange is hybridized to its complementary sequence, the thiazole orange acts as an intercalator. In addition to providing enhanced thermal stability, the dye adopts a mostly planar configuration resulting in significantly enhanced fluorescence. This “light up” effect can be as high as 34-fold depending on the sequence and how the dye is attached. This NHS ester will allow simple functionalization of internally located amino modifications such as those generated with amino-modifier C6 dT (10-1039).

Details

Usage

  • Coupling: Labeling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 5.4 mg of the Thiazole Orange NHS Ester in 200 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the Thiazole Orange NHS Ester solution and vortex. Let react for 15 min at room temperature and desalt.

 

Technical Documents

Safety Data Sheet

Glen Report 32.11: Thiazole Orange as a Fluorogenic Reporter in Oligonucleotide Probes

Glen Report 32.12: New Product ­—Thiazole Orange NHS Ester

 

Specifications
Diluent DMF or DMSO
Storage Freezer storage, -10 to -30°C, dry
Stability1-2 Days

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