Thiazole Orange NHS Ester
N-(5-carboxypentyl)-2-[(1,4-dihydro-1- methylquinolin-4-ylidene)methyl]benzothiazol-3-ium chloride N-hydroxysuccinimide ester
Description
Thiazole orange is an asymmetric
cyanine dye whose fluorescence can be quite dependent on its local
environment. When an oligonucleotide labeled with thiazole orange is
hybridized to its complementary sequence, the thiazole orange acts as an
intercalator. In addition to providing enhanced thermal stability, the
dye adopts a mostly planar configuration resulting in significantly
enhanced fluorescence. This “light up” effect can be as high as 34-fold
depending on the sequence and how the dye is attached. This NHS ester
will allow simple functionalization of internally located amino
modifications such as those generated with amino-modifier C6 dT (10-1039).
Details
Usage
- Coupling:
Labeling: For labeling 0.2 µmoles of an amino-modified oligonucleotide,
dissolve 5.4 mg of the Thiazole Orange NHS Ester in 200 µL DMSO (or
DMF). Dissolve the amino-modified oligonucleotide in 500 µL 0.1 M
carbonate/bicarbonate buffer (pH 9). Add 20 µL of the Thiazole Orange
NHS Ester solution and vortex. Let react for 15 min at room temperature
and desalt.
Safety Data Sheet
Glen Report 32.11: Thiazole Orange as a Fluorogenic Reporter in Oligonucleotide Probes
Glen Report 32.12: New Product —Thiazole Orange NHS Ester
| Specifications |
|---|
| Diluent |
DMF or DMSO |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 Days |
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