Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Plant

Specialised kits for isolating DNA from plant materials such as plant leaf, seeds, roots and stem.

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
QEP70750QuickExtract™ Plant DNA Extraction System500 Reactions £238.00£238.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).

The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.

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Figure 1. Overview of the QuickExtract™ Plant DNA extraction procedure.

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Figure 2. PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves. Forty cycles of RAPD were performed with DNA extracted from plant leaves using the QuickExtract Plant solution and the FailSafe™ PCR System. Lane M, 100-bp ladder; lane 1, pepper; lane 2, soybean; lane 3, spelt.
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Figure 3. Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. Six individual punches of four Arabidopsis leaves were treated with QuickExtract Plant solution as described in the product information sheet. One microliter of the solution was used in a 25-µl PCR using the FailSafe™ PCR System and primers specifi c for the single-copy HSC70 chromosomal gene. Aliquots were analyzed on a 2% agarose gel and the DNA was visualized by staining with SYBR® Gold. Lanes 1-6, PCR products of extracted DNA samples; lane 7, no-leaf negative control; lane M, 100-bp DNA ladder. The expected amplicon is approximately 520 bp

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

Protocols

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

 

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • High-throughput isolation of DNA from plant leaf samples for PCR-based analysis, e.g., GMO testing

Benefits

  • qPCR or PCR-ready sample.
  • No bead-beating, freezing, or grinding of plant leaf material.
  • Nontoxic, inexpensive processing.
  • Fast procedure (8 minutes for average sample).
  • No centrifugation or spin columns to reduce yields.
  • Suitable for high-throughput or automated workflows.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200