Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-6-Fluorescein Serinol CPG

3'-6-Fluorescein Serinol CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2994-013'-6-Fluorescein Serinol CPG 0.1g £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2994-103'-6-Fluorescein Serinol CPG 1.0g £796.00£732.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2994-133'-6-Fluorescein Serinol CPG 1x10µm £240.00£220.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2994-143'-6-Fluorescein Serinol CPG 1x15µm £360.00£331.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2994-413'-6-Fluorescein Serinol CPG 4x1.0µm £160.00£147.20Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2994-423'-6-Fluorescein Serinol CPG 4x0.2µm £96.00£88.32Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-6-Fluorescein Serinol CPG

3'-6-Fluorescein Serinol CPG

Glen Research

3'-6-Fluorescein Serinol CPG

Structure

Catalog Number: 20-2994-xx

Description: 3'-6-Fluorescein Serinol CPG

3-Dimethoxytrityloxy-2-(3-(6-carboxy-(di-O-pivaloyl-fluorescein)propanamido)propyl)-
1-O-succinyl-long chain alkylamino-CPG


F.W.: 584.47
Diluent: n/a
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Not Applicable

Serinol Reagents for Modification and Labelling

Most popular non-nucleosidic phosphoramidites for modification and labelling are based on two structural types: 1,2-diols and 1,3-diols. Products based on a 1,2-diol backbone were first described to allow amino-modification and biotin labelling. Technically, the 1,2-diol backbone has some drawbacks relative to the 1,3-diol backbone. The 1,2-diol backbone can participate in a dephosphorylation reaction since the 1,2-diol can form a favored 5-membered cyclic phosphate intermediate. This reaction is competitive with simple hydrolysis of the protecting groups and leads to some loss of label. However, the degree of loss at the 3’ terminus can be limited by the removal of the cyanoethyl protecting group using DBU or diethylamine prior to the cleavage and deprotection steps. Similarly, loss at the 5’ terminus can be eliminated by retaining the DMT group until the oligo is fully deprotected. Fortunately, the elimination reaction is virtually non-existent in the 1,3-diol backbone since the cyclic intermediate would be a 6-membered ring which is not favored for a cyclic phosphate intermediate.

IVD customers have requested a new backbone based on a 1,3-diol that would overcome any technical or IP issues surrounding our current products. We now offer a line of products based on the serinol backbone, which have been developed in close collaboration between Glen Research and Nelson Biotechnologies. Protected Biotin Serinol Phosphoramidite and CPG are protected with a t-butylbenzoyl group on the biotin ring. This group is designed to stop any phosphoramidite reactions at this active position in biotin. This protection avoids branching when using nucleophilic activators like DCI. The protecting group is easily removed during oligonucleotide cleavage and deprotection. The BiotinLC versions are similarly protected and should be useful for the synthesis of highly sensitive biotinylated probes. 6-Fluorescein Serinol Phosphoramidite and CPG are designed to prepare oligonucleotides containing one or several 6-Fluorescein (6-FAM) residues. Amino-Modifier Serinol Phosphoramidite and CPG are used to add amino groups into one or several positions in oligonucleotides. The amino group is protected with Fmoc, which may be removed on the synthesis column prior to solid-phase conjugation to the amino groups, or which may be removed during deprotection for subsequent solution phase conjugation to the amino groups.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-6-Fluorescein Serinol CPG

3'-6-Fluorescein Serinol CPG

Glen Research

MSDS

Glen Report 20.2

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200