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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-PT-Amino-Modifier C3 CPG

3'-PT-Amino-Modifier C3 CPG

Glen Research

3'-PT-Amino-Modifier C3 CPG

 

Structure

Catalog Number: 20-2954-xx

Description: 3'-PT-Amino-Modifier C3 CPG

N-(3-(O-Dimethoxytrityl)-propyl)-(2-carboxamide)-phthalimidyl-lcaa-CPG


F.W.: 137.07

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires overnight at 55°C with ammonium hydroxide or AMA for 10 minutes at 65°C. This treatment will complete the deprotection of the nucleobases. For a complete list of cleavage conditions, see www.glenres.com/GlenReports/GR15-12.html.
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: Not Applicable

3’-MODIFIERS

3’-Amino-Modifier CPGs, containing amino groups protected with the base-labile Fmoc group, are designed to functionalize the 3’-terminus of the target oligonucleotide by the introduction of a primary amine. In an alternative approach, the nitrogen destined to become the 3’-amino group is included in a phthalimide (PT) group which is attached to the support through an amide group attached to the aromatic ring. This simple linkage is very stable to all conditions of oligonucleotide synthesis and contains no chiral center. Using an extended ammonium hydroxide treatment (55°C for 17 hours), the cleavage of the amine from the phthalimide is accomplished along with the deprotection of the oligonucleotide. ABI-style columns are supplied unless otherwise requested.

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3'-PT-Amino-Modifier C3 CPG

3'-PT-Amino-Modifier C3 CPG

Glen Research

MSDS

Glen Report 15.1: Comparison of Deprotection Methods for 3'-PT-Amino-Modifier CPG

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200